Natriuretic peptides up-regulate aquaporin 3 in a human colonic epithelial cell line.

Itoh,Akihiko; Tsujikawa,Tomoyuki; Yasuoka,Takashi; Nakahara,Tamio; Sasaki,Masaya; Fujiyama,Yoshihide

doi:10.3892/ijmm.14.4.621

October 2004 Volume 14 Issue 4

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October 2004 Volume 14 Issue 4

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Article

Natriuretic peptides up-regulate aquaporin 3 in a human colonic epithelial cell line.

Authors:
- Akihiko Itoh
- Tomoyuki Tsujikawa
- Takashi Yasuoka
- Tamio Nakahara
- Masaya Sasaki
- Yoshihide Fujiyama
View Affiliations / Copyright

Affiliations: Division of Gastroenterology, Shiga University of Medical Science, Tsukinowa-cho, Seta, Otsu, Shiga 520-2192, Japan.
Pages: 621-627
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Published online on: October 1, 2004

https://doi.org/10.3892/ijmm.14.4.621
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Abstract

Several specialized channels termed aquaporins (AQPs) facilitate water transport in the gastrointestinal tract. AQP3 localizes to epithelial cells in the human small intestine and colon. However, the regulatory mechanisms responsible for AQP3 function in the gastrointestinal tract are not well understood. To characterize the regulation of AQP3 expression by atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), we studied mRNA expression by Northern blotting, protein expression by Western blotting and DNA binding activity by electrophoretic mobility shift assay (EMSA) in the human colonic epithelial cell line HT-29. We also used several inhibitors to investigate signal transduction. AQP3 mRNA was up-regulated in addition to ANP (>100 nM) and BNP (>10 nM). The expression of AQP3 protein was enhanced at 1 h after the addition of ANP and BNP. The combination of protein kinase-A (PK-A) and protein kinase-G (PK-G) inhibitors completely inhibited the expression of AQP3 mRNA enhanced by ANP or BNP to its basal level. The EMSA of the cyclic-AMP response element (CRE) in HT-29 cells revealed a single band. These results indicate that ANP and BNP up-regulated the expression of AQP3 mRNA and protein, and both PK-A and PK-G dependent pathways mediated this effect.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Natriuretic peptides up-regulate aquaporin 3 in a human colonic epithelial cell line.

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Abstract

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