Increase in metalloproteinase activity in the plasma of smoke-extract-injected rats.

Machida,Toshio; Kubota,Motoo; Saeki,Naokatsu; Takiguchi,Masaki; Yamaura,Akira; Hiwasa,Takaki

doi:10.3892/ijmm.14.4.659

October 2004 Volume 14 Issue 4

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October 2004 Volume 14 Issue 4

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Article

Increase in metalloproteinase activity in the plasma of smoke-extract-injected rats.

Authors:
- Toshio Machida
- Motoo Kubota
- Naokatsu Saeki
- Masaki Takiguchi
- Akira Yamaura
- Takaki Hiwasa
View Affiliations / Copyright

Affiliations: Department of Neurological Surgery, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chiba 260-8670, Japan.
Pages: 659-721
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Published online on: October 1, 2004

https://doi.org/10.3892/ijmm.14.4.659
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Abstract

Cigarette smoking is known to be one of the risk factors of subarachnoid hemorrhage (SAH), yet the precise mechanism remains to be proved. Based on the hypothesis that smoke components might enhance protease activity in the arterial wall, we examined the proteinase activities of plasma from smoke-extract-injected rats. Rats were injected intraperitoneally with smoke extract and the protease activity in the plasma was examined by zymography in the presence or the absence of proteinase inhibitors. Metalloproteinase activity was measured using a synthetic substrate. Proteolytic activities of 80- and 100-kDa against gelatin and collagen type I-V were induced 6 h after the injection. Inhibition of the activity by a metalloproteinase inhibitor but not by serine, cysteine or aspartic protease inhibitors suggested that the proteinases can be attributed to metalloproteinases. Cleavage activity in plasma toward a synthetic metalloproteinase substrate also increased within 24 h after the injection of the smoke extract. The results indicate that the induction of plasma metalloproteinase activity by smoke extract might account for the causal role of smoking in the development of SAH.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Increase in metalloproteinase activity in the plasma of smoke-extract-injected rats.

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