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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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October 2004 Volume 14 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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October 2004 Volume 14 Issue 4

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Article

Measurement of antibody-membrane interactions by surface plasmon resonance.

  • Authors:
    • Alexander Klimka
    • Mehmet K Tur
    • Michael Huhn
    • Uwe Bierfreund
    • Estela Terrada
    • Rainer Fischer
    • Ricarda Finnern
    • Stefan Barth
  • View Affiliations / Copyright

    Affiliations: Department of Pharmaceutical Product Development, Fraunhofer IME, Worringerweg 1, 52074 Aachen, Germany.
  • Pages: 765-773
    |
    Published online on: October 1, 2004
       https://doi.org/10.3892/ijmm.14.4.765
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Abstract

Due to problems of immobilizing functional tumor antigens in their natural conformation on surfaces for immunoassays, it is often difficult to evaluate the binding of antibodies derived from phage display libraries depleted and selected by panning on cell lines and living tumor cells. Performing cell membrane based ELISA methods does not reveal any up front kinetic binding information and depends on the performance of secondary antibodies and substrates. To overcome these limitations, we developed a new method to visualize direct antibody-cell membrane interactions by surface plasmon resonance using the Biacore 3000 and on-line signal subtraction on antigen-negative cell membrane vesicles. Conditions for the coating of cell membrane preparations to a carboxymethyl dextran hydrogel surface of a commercially available chip and the proof of concept for this application by the analysis of different formats of anti-CD30 and anti-carcinoembryonic antigen (CEA) antibodies interacting with coated membrane vesicles of CD30-positive/CEA-negative and CD30-negative and CEA-positive cell lines are described.

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Copy and paste a formatted citation
Spandidos Publications style
Klimka A, Tur M, Huhn M, Bierfreund U, Terrada E, Fischer R, Finnern R and Barth S: Measurement of antibody-membrane interactions by surface plasmon resonance.. Int J Mol Med 14: 765-773, 2004.
APA
Klimka, A., Tur, M., Huhn, M., Bierfreund, U., Terrada, E., Fischer, R. ... Barth, S. (2004). Measurement of antibody-membrane interactions by surface plasmon resonance.. International Journal of Molecular Medicine, 14, 765-773. https://doi.org/10.3892/ijmm.14.4.765
MLA
Klimka, A., Tur, M., Huhn, M., Bierfreund, U., Terrada, E., Fischer, R., Finnern, R., Barth, S."Measurement of antibody-membrane interactions by surface plasmon resonance.". International Journal of Molecular Medicine 14.4 (2004): 765-773.
Chicago
Klimka, A., Tur, M., Huhn, M., Bierfreund, U., Terrada, E., Fischer, R., Finnern, R., Barth, S."Measurement of antibody-membrane interactions by surface plasmon resonance.". International Journal of Molecular Medicine 14, no. 4 (2004): 765-773. https://doi.org/10.3892/ijmm.14.4.765
Copy and paste a formatted citation
x
Spandidos Publications style
Klimka A, Tur M, Huhn M, Bierfreund U, Terrada E, Fischer R, Finnern R and Barth S: Measurement of antibody-membrane interactions by surface plasmon resonance.. Int J Mol Med 14: 765-773, 2004.
APA
Klimka, A., Tur, M., Huhn, M., Bierfreund, U., Terrada, E., Fischer, R. ... Barth, S. (2004). Measurement of antibody-membrane interactions by surface plasmon resonance.. International Journal of Molecular Medicine, 14, 765-773. https://doi.org/10.3892/ijmm.14.4.765
MLA
Klimka, A., Tur, M., Huhn, M., Bierfreund, U., Terrada, E., Fischer, R., Finnern, R., Barth, S."Measurement of antibody-membrane interactions by surface plasmon resonance.". International Journal of Molecular Medicine 14.4 (2004): 765-773.
Chicago
Klimka, A., Tur, M., Huhn, M., Bierfreund, U., Terrada, E., Fischer, R., Finnern, R., Barth, S."Measurement of antibody-membrane interactions by surface plasmon resonance.". International Journal of Molecular Medicine 14, no. 4 (2004): 765-773. https://doi.org/10.3892/ijmm.14.4.765
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