Identification of differentiation-inducing activity produced by human bone marrow stromal cell line LP101

Hiramoto,Masaki; Kawakami,Yutaka; Nabeshima,Ryusuke; Shima,Daisuke; Handa,Hiroshi; Aizawa,Shin

doi:10.3892/ijmm.14.5.867

November 2004 Volume 14 Issue 5

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November 2004 Volume 14 Issue 5

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Article

Identification of differentiation-inducing activity produced by human bone marrow stromal cell line LP101

Authors:
- Masaki Hiramoto
- Yutaka Kawakami
- Ryusuke Nabeshima
- Daisuke Shima
- Hiroshi Handa
- Shin Aizawa
View Affiliations / Copyright

Affiliations: Department of Anatomy, Nihon University School of Medicine, Tokyo, Japan
Pages: 867-872
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Published online on: November 1, 2004

https://doi.org/10.3892/ijmm.14.5.867
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Abstract

We have previously reported that human promyelocytic leukemia HL-60 cells can be induced to differentiate into mature granulocytes when HL-60 co-cultivated with human bone marrow stromal LP101 cells. In the present study, we investigated which factors produced by LP101 cells induce HL-60 cells to differentiate into mature granulocytes. The expression of the cell surface antigen CD11b on HL-60 cells was increased after a 72-h culture with the conditioned medium (CM) obtained from LP101 cells. LP101 cells were observed to produce various cytokines, including TNF-α, GM-CSF and IL-6. The neutralizing antibodies against these cytokines partially suppressed the CM-induced differentiation of HL-60 cells. Recombinant TNF-α induced the differentiation of HL-60 cells, and GM-CSF and IL-6 additionally enhanced the effect of TNF-α. When the CM was divided into a low molecular weight (LMW) fraction and a high molecular weight (HMW) fraction by ultrafiltration, the LMW fraction synergistically enhanced the differentiation inducible activity of TNF-α. These results demonstrate that LP101 cells induce the differentiation of HL-60 cells by producing various cytokines including TNF-α, IL-6, and GM-CSF, and that unknown low molecular weight factors also participate.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Identification of differentiation-inducing activity produced by human bone marrow stromal cell line LP101

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Abstract

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