Interleukin-5, interleukin-3 and granulocyte-macrophage colony-stimulating factor prime actin-polymerization in human eosinophils: A study with hypodense and normodense eosinophils from patients with atopic dermatitis

  • Authors:
    • Martin Kaatz
    • Luciana Berod
    • Wolfgang Czech
    • Marco Idzko
    • Mariana Lagadari
    • Andrea Bauer
    • Johannes Norgauer
  • View Affiliations

  • Published online on: December 1, 2004     https://doi.org/10.3892/ijmm.14.6.1055
  • Pages: 1055-1060
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Characteristic features of atopic diseases (AD) are immigration and local activation of eosinophils. Reorganization of the cytoskeleton modulates the function of leukocytes and is a prerequisite for the motility response. In this work, the regulation of actin polymerization has been investigated by flow cytometry using NBD-phallacidin and right angle light scatter measurements in purified eosinophils isolated from patients with atopic dermatitis and normal individuals. Stimulation of eosinophils with chemotaxins such as complement fragment C5a (C5a), CC chemokine RANTES/ CCL5 and platelet activating factor (PAF) induced a reversible polymerization of actin. Normodense eosinophils purified from patients with AD showed a decreased chemotaxin-induced actin response as compared to normodense eosinophils from healthy subjects and hypodense eosinophils from patients. Stimulation of eosinophils with Th2-cytokines such as interleukin-3 (IL-3), interleukin-5 (IL-5), granulocyte-macrophage colony-stimulating factor (GM-CSF) did not exert a significant effect on actin polymerization. However, pretreatment with IL-3, IL-5 or GM-CSF potentiated the chemotaxin-induced actin polymerization and graded the differential responsiveness between normodense and hypodense eosinophils. We demonstrate a different actin responsiveness in eosinophils from atopic patients and healthy subjects which could be overcome by modulating effects of Th2-cytokines.

Related Articles

Journal Cover

December 2004
Volume 14 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kaatz M, Berod L, Czech W, Idzko M, Lagadari M, Bauer A and Norgauer J: Interleukin-5, interleukin-3 and granulocyte-macrophage colony-stimulating factor prime actin-polymerization in human eosinophils: A study with hypodense and normodense eosinophils from patients with atopic dermatitis. Int J Mol Med 14: 1055-1060, 2004
APA
Kaatz, M., Berod, L., Czech, W., Idzko, M., Lagadari, M., Bauer, A., & Norgauer, J. (2004). Interleukin-5, interleukin-3 and granulocyte-macrophage colony-stimulating factor prime actin-polymerization in human eosinophils: A study with hypodense and normodense eosinophils from patients with atopic dermatitis. International Journal of Molecular Medicine, 14, 1055-1060. https://doi.org/10.3892/ijmm.14.6.1055
MLA
Kaatz, M., Berod, L., Czech, W., Idzko, M., Lagadari, M., Bauer, A., Norgauer, J."Interleukin-5, interleukin-3 and granulocyte-macrophage colony-stimulating factor prime actin-polymerization in human eosinophils: A study with hypodense and normodense eosinophils from patients with atopic dermatitis". International Journal of Molecular Medicine 14.6 (2004): 1055-1060.
Chicago
Kaatz, M., Berod, L., Czech, W., Idzko, M., Lagadari, M., Bauer, A., Norgauer, J."Interleukin-5, interleukin-3 and granulocyte-macrophage colony-stimulating factor prime actin-polymerization in human eosinophils: A study with hypodense and normodense eosinophils from patients with atopic dermatitis". International Journal of Molecular Medicine 14, no. 6 (2004): 1055-1060. https://doi.org/10.3892/ijmm.14.6.1055