Induction of apoptosis by dideoxypetrosynol A, a polyacetylene from the sponge Petrosia sp., in human skin melanoma cells

  • Authors:
    • Hye Joung Choi
    • Song-Ja Bae
    • Nam Deuk Kim
    • Jee H. Jung
    • Yung Hyun Choi
  • View Affiliations

  • Published online on: December 1, 2004     https://doi.org/10.3892/ijmm.14.6.1091
  • Pages: 1091-1096
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Abstract

Dideoxypetrosynol A, a polyacetylene from the sponge Petrosia sp., is known to exhibit significant selective cytotoxicity against several human tumor cell lines. In the present study, we investigated the possible mechanisms by which dideoxypetrosynol A exerts its anti-proliferative action in cultured human SK-MEL-2 skin melanoma cells. Exposure of SK-MEL-2 cells to dideoxypetrosynol A resulted in growth inhibition and induction of apoptosis in a dose-dependent manner as measured by MTT assay, fluorescent microscopy and flow cytometry analysis. The increase in apoptosis was associated with a dose-dependent up-regulation in proapoptotic Bax expression and down-regulation of anti-apoptotic Bcl-2. Apoptosis-inducing concentrations of dideoxypetrosynol A induced caspase-3 and caspase-9 activation accompanied by proteolytic degradation of poly(ADP-ribose)-polymerase and selective down-regulation of cIAP-1. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of dideoxypetrosynol A.

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December 2004
Volume 14 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Choi HJ, Bae S, Kim ND, Jung JH and Choi YH: Induction of apoptosis by dideoxypetrosynol A, a polyacetylene from the sponge Petrosia sp., in human skin melanoma cells. Int J Mol Med 14: 1091-1096, 2004
APA
Choi, H.J., Bae, S., Kim, N.D., Jung, J.H., & Choi, Y.H. (2004). Induction of apoptosis by dideoxypetrosynol A, a polyacetylene from the sponge Petrosia sp., in human skin melanoma cells. International Journal of Molecular Medicine, 14, 1091-1096. https://doi.org/10.3892/ijmm.14.6.1091
MLA
Choi, H. J., Bae, S., Kim, N. D., Jung, J. H., Choi, Y. H."Induction of apoptosis by dideoxypetrosynol A, a polyacetylene from the sponge Petrosia sp., in human skin melanoma cells". International Journal of Molecular Medicine 14.6 (2004): 1091-1096.
Chicago
Choi, H. J., Bae, S., Kim, N. D., Jung, J. H., Choi, Y. H."Induction of apoptosis by dideoxypetrosynol A, a polyacetylene from the sponge Petrosia sp., in human skin melanoma cells". International Journal of Molecular Medicine 14, no. 6 (2004): 1091-1096. https://doi.org/10.3892/ijmm.14.6.1091