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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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January 2005 Volume 15 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

The kinase domain of death-associated protein kinase is inhibitory for tubulointerstitial fibrosis in chronic obstructive nephropathy

  • Authors:
    • Kazunori Yukawa
    • Masanori Kishino
    • Katsuaki Hoshino
    • Nobuyuki Shirasawa
    • Akihiko Kimura
    • Yuji Tsubota
    • Kyoko Owada-Makabe
    • Tao Bai
    • Tetsuji Tanaka
    • Takashi Ueyama
    • Masakazu Ichinose
    • Kiyoshi Takeda
    • Shizuo Akira
    • Masanobu Maeda
  • View Affiliations / Copyright

    Affiliations: Department of Physiology, Wakayama Medical University, Wakayama 641-8509, Japan. kazu59@wakayama-med.ac.jp
  • Pages: 73-78
    |
    Published online on: January 1, 2005
       https://doi.org/10.3892/ijmm.15.1.73
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Abstract

Death-associated protein kinase (DAPK) is a Ca2+/calmodulin-dependent serine/threonine kinase that is thought to mediate apoptosis. We have shown that the kinase domain of DAPK is crucial for the induction of renal tubular cell apoptosis in chronic obstructive uropathy (COU) created by unilateral ureteral ligation. DAPK-mutant mice, generated by deletion of 74 amino acids from the catalytic kinase domain, were used to investigate the role of the DAPK kinase domain in renal fibrosis following COU. Interstitial collagen and α-smooth muscle actin (α-SMA) expressions in situ were compared between obstructed kidneys in wild-type and mutant mice. As a result, tubulointerstitial fibrosis, as quantified by interstitial collagen expression, was significantly augmented in mutant kidneys compared with wild-type kidneys following COU. Furthermore, deletion of the kinase domain from DAPK significantly increased the appearance of α-SMA-positive myofibroblasts in the renal interstitium during COU. Thus, our results suggest that the kinase domain deleted by gene targeting plays a suppressive role for the development of renal fibrosis through inhibition of the tubular epithelial-to-mesenchymal transition in a mouse model of COU.

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Copy and paste a formatted citation
Spandidos Publications style
Yukawa K, Kishino M, Hoshino K, Shirasawa N, Kimura A, Tsubota Y, Owada-Makabe K, Bai T, Tanaka T, Ueyama T, Ueyama T, et al: The kinase domain of death-associated protein kinase is inhibitory for tubulointerstitial fibrosis in chronic obstructive nephropathy. Int J Mol Med 15: 73-78, 2005.
APA
Yukawa, K., Kishino, M., Hoshino, K., Shirasawa, N., Kimura, A., Tsubota, Y. ... Maeda, M. (2005). The kinase domain of death-associated protein kinase is inhibitory for tubulointerstitial fibrosis in chronic obstructive nephropathy. International Journal of Molecular Medicine, 15, 73-78. https://doi.org/10.3892/ijmm.15.1.73
MLA
Yukawa, K., Kishino, M., Hoshino, K., Shirasawa, N., Kimura, A., Tsubota, Y., Owada-Makabe, K., Bai, T., Tanaka, T., Ueyama, T., Ichinose, M., Takeda, K., Akira, S., Maeda, M."The kinase domain of death-associated protein kinase is inhibitory for tubulointerstitial fibrosis in chronic obstructive nephropathy". International Journal of Molecular Medicine 15.1 (2005): 73-78.
Chicago
Yukawa, K., Kishino, M., Hoshino, K., Shirasawa, N., Kimura, A., Tsubota, Y., Owada-Makabe, K., Bai, T., Tanaka, T., Ueyama, T., Ichinose, M., Takeda, K., Akira, S., Maeda, M."The kinase domain of death-associated protein kinase is inhibitory for tubulointerstitial fibrosis in chronic obstructive nephropathy". International Journal of Molecular Medicine 15, no. 1 (2005): 73-78. https://doi.org/10.3892/ijmm.15.1.73
Copy and paste a formatted citation
x
Spandidos Publications style
Yukawa K, Kishino M, Hoshino K, Shirasawa N, Kimura A, Tsubota Y, Owada-Makabe K, Bai T, Tanaka T, Ueyama T, Ueyama T, et al: The kinase domain of death-associated protein kinase is inhibitory for tubulointerstitial fibrosis in chronic obstructive nephropathy. Int J Mol Med 15: 73-78, 2005.
APA
Yukawa, K., Kishino, M., Hoshino, K., Shirasawa, N., Kimura, A., Tsubota, Y. ... Maeda, M. (2005). The kinase domain of death-associated protein kinase is inhibitory for tubulointerstitial fibrosis in chronic obstructive nephropathy. International Journal of Molecular Medicine, 15, 73-78. https://doi.org/10.3892/ijmm.15.1.73
MLA
Yukawa, K., Kishino, M., Hoshino, K., Shirasawa, N., Kimura, A., Tsubota, Y., Owada-Makabe, K., Bai, T., Tanaka, T., Ueyama, T., Ichinose, M., Takeda, K., Akira, S., Maeda, M."The kinase domain of death-associated protein kinase is inhibitory for tubulointerstitial fibrosis in chronic obstructive nephropathy". International Journal of Molecular Medicine 15.1 (2005): 73-78.
Chicago
Yukawa, K., Kishino, M., Hoshino, K., Shirasawa, N., Kimura, A., Tsubota, Y., Owada-Makabe, K., Bai, T., Tanaka, T., Ueyama, T., Ichinose, M., Takeda, K., Akira, S., Maeda, M."The kinase domain of death-associated protein kinase is inhibitory for tubulointerstitial fibrosis in chronic obstructive nephropathy". International Journal of Molecular Medicine 15, no. 1 (2005): 73-78. https://doi.org/10.3892/ijmm.15.1.73
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