Influence of glucose and inflammatory cytokines on TGF-β1 and CTGF mRNA expressions in human peritoneal mesothelial cells

  • Authors:
    • Nobuyoshi Sakamoto
    • Kazunobu Sugimura
    • Hidenori Kawashima
    • Kenji Tsuchida
    • Yoshiaki Takemoto
    • Toshihide Naganuma
    • Shinji Tatsumi
    • Tatsuya Nakatani
  • View Affiliations

  • Published online on: June 1, 2005     https://doi.org/10.3892/ijmm.15.6.907
  • Pages: 907-911
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Abstract

Peritoneal fibrosis is a major complication of long-term continuous ambulatory peritoneal dialysis (CAPD) treatment. Transforming growth factor-β (TGF-β) has been reported to play an important role in the fibrosis of various tissues by stimulating connective tissue growth factor (CTGF) expression. In order to elucidate the mechanism of CAPD-related peritoneal fibrosis, we studied the influence of high glucose concentrations and inflammatory cytokines on mRNA expressions of TGF-β and CTGF in cultured human peritoneal mesothelial cells (HPMC). HPMC were isolated from normal omentum and cultured with 0.5 or 1.0% glucose or mannitol for 7 days. TGF-β1 and CTGF mRNA were quantified by one step real-time reverse transcription-polymerase chain reaction (real-time RT-PCR). TGF-β1 and CTGF mRNA expression levels were significantly increased (p<0.05) by glucose in a dose-dependent manner, but not by mannitol. The expression levels were correlated between TGF-β1 and CTGF. Effects of inflammatory cytokines were also examined by adding tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1) or interleukin-6 (IL-6) to the medium at 0.1 ng/ml for 2 days. TGF-β1 expression tended to be increased by TNF-α and IL-6. On the other hand, CTGF expression was significantly decreased (p<0.01) by IL-1 but not changed by TNF-α or IL-6. These results suggest that high glucose concentration may play a central role in peritoneal fibrosis. Responses of TGF-β1 and CTGF to inflammatory cytokines were not necessarily identical, suggesting that CTGF may be a better therapeutic target for peritoneal fibrosis than TGF-β1.

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June 2005
Volume 15 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sakamoto N, Sugimura K, Kawashima H, Tsuchida K, Takemoto Y, Naganuma T, Tatsumi S and Nakatani T: Influence of glucose and inflammatory cytokines on TGF-β1 and CTGF mRNA expressions in human peritoneal mesothelial cells. Int J Mol Med 15: 907-911, 2005
APA
Sakamoto, N., Sugimura, K., Kawashima, H., Tsuchida, K., Takemoto, Y., Naganuma, T. ... Nakatani, T. (2005). Influence of glucose and inflammatory cytokines on TGF-β1 and CTGF mRNA expressions in human peritoneal mesothelial cells. International Journal of Molecular Medicine, 15, 907-911. https://doi.org/10.3892/ijmm.15.6.907
MLA
Sakamoto, N., Sugimura, K., Kawashima, H., Tsuchida, K., Takemoto, Y., Naganuma, T., Tatsumi, S., Nakatani, T."Influence of glucose and inflammatory cytokines on TGF-β1 and CTGF mRNA expressions in human peritoneal mesothelial cells". International Journal of Molecular Medicine 15.6 (2005): 907-911.
Chicago
Sakamoto, N., Sugimura, K., Kawashima, H., Tsuchida, K., Takemoto, Y., Naganuma, T., Tatsumi, S., Nakatani, T."Influence of glucose and inflammatory cytokines on TGF-β1 and CTGF mRNA expressions in human peritoneal mesothelial cells". International Journal of Molecular Medicine 15, no. 6 (2005): 907-911. https://doi.org/10.3892/ijmm.15.6.907