Evidence that orexins A and B stimulate insulin secretion from rat pancreatic islets via both receptor subtypes

  • Authors:
    • Krzysztof W. Nowak
    • Mathias Z. Strowski
    • Malgorzata M. Switonska
    • Przemyslaw Kaczmarek
    • Vandana Singh
    • Marzena Fabis
    • Pawel Mackowiak
    • Magdalena Nowak
    • Ludwik K. Malendowicz
  • View Affiliations

  • Published online on: June 1, 2005     https://doi.org/10.3892/ijmm.15.6.969
  • Pages: 969-972
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Abstract

Orexins are recently identified neuropeptides that appear to play a role in the regulation of energy homeostasis and arousal. They bind to and activate two closely related G protein-coupled receptors (OXR1 and OXR2), previously described as orphans. In this study we examined involvement of orexins in regulation of insulin secretion from rat pancreatic islets utilizing an in situ perfused pancreas and isolated pancreatic islet models. By means of RT-PCR we found that both OXR1 and OXR2 are expressed in rat pancreatic islets. Furthermore, the expression levels of OXR1 were higher than OXR2. In both experimental models applied, orexins A and B (1, 10 and 100 nmol/l) concentration dependently stimulated insulin secretion at two different glucose concentrations (6.66 or 26.4 mmol/l), with orexin A being more potent than orexin B. This study demonstrates that orexins A and B modulate insulin secretion in vitro.

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June 2005
Volume 15 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Nowak KW, Strowski MZ, Switonska MM, Kaczmarek P, Singh V, Fabis M, Mackowiak P, Nowak M and Malendowicz LK: Evidence that orexins A and B stimulate insulin secretion from rat pancreatic islets via both receptor subtypes. Int J Mol Med 15: 969-972, 2005
APA
Nowak, K.W., Strowski, M.Z., Switonska, M.M., Kaczmarek, P., Singh, V., Fabis, M. ... Malendowicz, L.K. (2005). Evidence that orexins A and B stimulate insulin secretion from rat pancreatic islets via both receptor subtypes. International Journal of Molecular Medicine, 15, 969-972. https://doi.org/10.3892/ijmm.15.6.969
MLA
Nowak, K. W., Strowski, M. Z., Switonska, M. M., Kaczmarek, P., Singh, V., Fabis, M., Mackowiak, P., Nowak, M., Malendowicz, L. K."Evidence that orexins A and B stimulate insulin secretion from rat pancreatic islets via both receptor subtypes". International Journal of Molecular Medicine 15.6 (2005): 969-972.
Chicago
Nowak, K. W., Strowski, M. Z., Switonska, M. M., Kaczmarek, P., Singh, V., Fabis, M., Mackowiak, P., Nowak, M., Malendowicz, L. K."Evidence that orexins A and B stimulate insulin secretion from rat pancreatic islets via both receptor subtypes". International Journal of Molecular Medicine 15, no. 6 (2005): 969-972. https://doi.org/10.3892/ijmm.15.6.969