Increased myocardial N-myristoyltransferase activity in rotenone model of Parkinsonism

  • Authors:
    • Mohammed Khysar Pasha
    • Rajendra K. Sharma
    • Alexander H. Rajput
  • View Affiliations

  • Published online on: June 1, 2005     https://doi.org/10.3892/ijmm.15.6.987
  • Pages: 987-991
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Abstract

There is widespread brain pathology in Parkinson's disease (PD), with the primary pathology in the substantia nigra. Oxidative stress is believed to play a role in cell death in PD. Rotenone is a mitochondrial toxin which can produce Parkinson syndrome (PS) in rats. Myristoyl-CoA:protein N-myristoyltransferase (NMT), which catalyzes the co-translational transfer of myristate from myristoyl-CoA to the amino-terminal glycine residue of selected polypeptides, is increased in the myocardium of ischemia-reperfusion rat model myocardium. Animals received rotoneone (n=10) or placebo vehicle (n=6) via Alzet™ osmotic pumps. Mean cardiac muscle NMT activity of placebo treated (control) rats was 0.608±0.366 units/mg protein. Rats with mild or no detectable PS features on rotenone showed slight (mean 0.853±0.192) but insignificantly increased activity. Rats that had moderately severe PS features had higher level of NMT activity (mean 1.223±0.057), which was borderline significant compared to controls (P=0.066). Rats with severe PS features had the highest NMT activity (1.353±0.128) which was significantly greater compared to controls (P=0.003) and to the rats that had equivocal or no motor slowing (P=0.005). Our data show cardiac metabolic dysfunction in a rotenone rat model of PS. The severity of this change correlates with the severity of motor manifestations. Further studies of NMT activity in human PD cases and patients with cardiomyopathy of unknown cause may provide valuable information in these disorders.

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June 2005
Volume 15 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Pasha MK, Sharma RK and Rajput AH: Increased myocardial N-myristoyltransferase activity in rotenone model of Parkinsonism. Int J Mol Med 15: 987-991, 2005
APA
Pasha, M.K., Sharma, R.K., & Rajput, A.H. (2005). Increased myocardial N-myristoyltransferase activity in rotenone model of Parkinsonism. International Journal of Molecular Medicine, 15, 987-991. https://doi.org/10.3892/ijmm.15.6.987
MLA
Pasha, M. K., Sharma, R. K., Rajput, A. H."Increased myocardial N-myristoyltransferase activity in rotenone model of Parkinsonism". International Journal of Molecular Medicine 15.6 (2005): 987-991.
Chicago
Pasha, M. K., Sharma, R. K., Rajput, A. H."Increased myocardial N-myristoyltransferase activity in rotenone model of Parkinsonism". International Journal of Molecular Medicine 15, no. 6 (2005): 987-991. https://doi.org/10.3892/ijmm.15.6.987