Regulatory effects of emodin on NF-κB activation and inflammatory cytokine expression in RAW 264.7 macrophages

  • Authors:
    • Hai-Long Li
    • Hai-Long Chen
    • Hong Li
    • Kai-Li Zhang
    • Xiao-Yan Chen
    • Xiao-Wei Wang
    • Qing-You Kong
    • Jia Liu
  • View Affiliations

  • Published online on: July 1, 2005     https://doi.org/10.3892/ijmm.16.1.41
  • Pages: 41-47
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Abstract

Emodin, an anthraquinones component of Rheum palmatun, has been used for anti-inflammatory purposes. However, its underlying molecular effect(s) on target cells remain to be well clarified. Thus, our current study was aimed at investigating the regulatory mechanism of emodin on liposaccharide-induced inflammatory responses in RAW 264.7 macrophages by RT-PCR, Western blot analysis, immunocytochemical staining and immunofluorescence analysis. It was found that a treatment of 20 µg/ml emodin inhibited the expression of a panel of inflammatory-associated genes, including TNFα, iNOS, IL-10, cytosolic IκBα, IKK-α and IKK-γ, to different extents as well as the nuclear translocation of NF-κB (nuclear factor-κB). The promoting effect of emodin on the production and translocation of p105 (the precursor of NF-κB p50) was time-dependent and reached a maximum at 5 h. Our data suggest that emodin plays its anti-inflammatory roles by regulating inflammatory cytokines, specifically by suppressing NF-κB activation.

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July 2005
Volume 16 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li H, Chen H, Li H, Zhang K, Chen X, Wang X, Kong Q and Liu J: Regulatory effects of emodin on NF-κB activation and inflammatory cytokine expression in RAW 264.7 macrophages. Int J Mol Med 16: 41-47, 2005.
APA
Li, H., Chen, H., Li, H., Zhang, K., Chen, X., Wang, X. ... Liu, J. (2005). Regulatory effects of emodin on NF-κB activation and inflammatory cytokine expression in RAW 264.7 macrophages. International Journal of Molecular Medicine, 16, 41-47. https://doi.org/10.3892/ijmm.16.1.41
MLA
Li, H., Chen, H., Li, H., Zhang, K., Chen, X., Wang, X., Kong, Q., Liu, J."Regulatory effects of emodin on NF-κB activation and inflammatory cytokine expression in RAW 264.7 macrophages". International Journal of Molecular Medicine 16.1 (2005): 41-47.
Chicago
Li, H., Chen, H., Li, H., Zhang, K., Chen, X., Wang, X., Kong, Q., Liu, J."Regulatory effects of emodin on NF-κB activation and inflammatory cytokine expression in RAW 264.7 macrophages". International Journal of Molecular Medicine 16, no. 1 (2005): 41-47. https://doi.org/10.3892/ijmm.16.1.41