Preparation of anti-orotate phosphoribosyltransferase antibody and its application to immunochemical detection in human tumor cells

  • Authors:
    • Kazuki Sakamoto
    • Yoshikazu Sugimoto
    • Kazutaka Miyadera
    • Toshinori Oka
    • Masakazu Fukushima
  • View Affiliations

  • Published online on: August 1, 2005     https://doi.org/10.3892/ijmm.16.2.245
  • Pages: 245-249
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Abstract

Orotate phosphoribosyltransferase (OPRT) is a key enzyme in the anabolism of 5-fluorouracil (5-FU), and its expression in tumors is thought to increase the efficacy of 5-FU against the tumor. To detect the OPRT protein by immunoblot and/or immunohistochemical methods, we prepared highly specific antibody to the peptides contained in the human OPRT amino acid sequence. The anti-OPRT polyclonal antibodies, obtained by immunizing rabbits with the OPRT peptides, had a high specificity for the OPRT protein in human tumor xenografts when it was analyzed by immunoblotting, and furthermore, there was a positive correlation between OPRT activity and protein content in 12 human tumors (R2=0.632). These results suggest that immunohistochemical detection of tumoral OPRT protein expression with our anti-OPRT antibodies may provide a useful method for predicting the clinical response to 5-FU-based chemotherapy.

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August 2005
Volume 16 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sakamoto K, Sugimoto Y, Miyadera K, Oka T and Fukushima M: Preparation of anti-orotate phosphoribosyltransferase antibody and its application to immunochemical detection in human tumor cells. Int J Mol Med 16: 245-249, 2005.
APA
Sakamoto, K., Sugimoto, Y., Miyadera, K., Oka, T., & Fukushima, M. (2005). Preparation of anti-orotate phosphoribosyltransferase antibody and its application to immunochemical detection in human tumor cells. International Journal of Molecular Medicine, 16, 245-249. https://doi.org/10.3892/ijmm.16.2.245
MLA
Sakamoto, K., Sugimoto, Y., Miyadera, K., Oka, T., Fukushima, M."Preparation of anti-orotate phosphoribosyltransferase antibody and its application to immunochemical detection in human tumor cells". International Journal of Molecular Medicine 16.2 (2005): 245-249.
Chicago
Sakamoto, K., Sugimoto, Y., Miyadera, K., Oka, T., Fukushima, M."Preparation of anti-orotate phosphoribosyltransferase antibody and its application to immunochemical detection in human tumor cells". International Journal of Molecular Medicine 16, no. 2 (2005): 245-249. https://doi.org/10.3892/ijmm.16.2.245