Repetitive and safe transgene expression in rat liver is achievable by adenoviral infusion into the common bile duct

  • Authors:
    • Shigeki Kuriyama
    • Hitoshi Yoshiji
    • Kentaro Tominaga
    • Hirohisa Tsujinoue
    • Toshiya Nakatani
    • Tatsuhiro Tsujimoto
    • Hirotsugu Okuda
    • Takemi Akahane
    • Akihiro Deguchi
    • Seiji Nakai
    • Tsutomu Masaki
    • Naohito Uchida
  • View Affiliations

  • Published online on: October 1, 2005     https://doi.org/10.3892/ijmm.16.4.503
  • Pages: 503-508
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Abstract

To examine the feasibility of adenovirus-mediated gene transfer into the liver, we examined whether adenoviral infusion into the common bile duct could induce repetitive and safe transgene expression in rat livers. Recombinant adenovirus carrying a reporter lacZ gene was repetitively infused retrogradely into the common bile duct of rats. LacZ expression in rat livers was estimated histochemically by X-gal staining and quantitatively by a chemiluminescent reporter gene assay after the first, second and third adenoviral infusion into the common bile duct. To assess the safety of repetitive adenoviral infusion into the common bile duct, various liver- and kidney-related serum parameters, and liver damage were examined biochemically and histologically, respectively. Retrograde adenoviral infusion into the common bile duct achieved sufficient and safe lacZ expression in rat livers. Although transgene expression in the liver was transient, the second and third adenoviral infusion into the common bile duct could induce the expression of the same transgene in the liver. Furthermore, repetitive adenoviral infusion into the common bile duct caused no significant reverse reactions. Because retrograde adenoviral infusion into the common bile duct is a clinically practical method by using a widely used endoscopic technique, namely endoscopic retrograde cholangiography, these results suggest that retrograde adenoviral infusion into the common bile duct is a practical gene therapy modality in clinical settings.

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October 2005
Volume 16 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kuriyama S, Yoshiji H, Tominaga K, Tsujinoue H, Nakatani T, Tsujimoto T, Okuda H, Akahane T, Deguchi A, Nakai S, Nakai S, et al: Repetitive and safe transgene expression in rat liver is achievable by adenoviral infusion into the common bile duct. Int J Mol Med 16: 503-508, 2005.
APA
Kuriyama, S., Yoshiji, H., Tominaga, K., Tsujinoue, H., Nakatani, T., Tsujimoto, T. ... Uchida, N. (2005). Repetitive and safe transgene expression in rat liver is achievable by adenoviral infusion into the common bile duct. International Journal of Molecular Medicine, 16, 503-508. https://doi.org/10.3892/ijmm.16.4.503
MLA
Kuriyama, S., Yoshiji, H., Tominaga, K., Tsujinoue, H., Nakatani, T., Tsujimoto, T., Okuda, H., Akahane, T., Deguchi, A., Nakai, S., Masaki, T., Uchida, N."Repetitive and safe transgene expression in rat liver is achievable by adenoviral infusion into the common bile duct". International Journal of Molecular Medicine 16.4 (2005): 503-508.
Chicago
Kuriyama, S., Yoshiji, H., Tominaga, K., Tsujinoue, H., Nakatani, T., Tsujimoto, T., Okuda, H., Akahane, T., Deguchi, A., Nakai, S., Masaki, T., Uchida, N."Repetitive and safe transgene expression in rat liver is achievable by adenoviral infusion into the common bile duct". International Journal of Molecular Medicine 16, no. 4 (2005): 503-508. https://doi.org/10.3892/ijmm.16.4.503