Failure of interferon-γ and tumor necrosis factor in mediating anemia of chronic disease in a mouse model of protracted septic peritonitis

  • Authors:
    • Thomas E.O. Schubert
    • Bernd Echtenacher
    • Ferdinand Hofstädter
    • Daniela N. Männel
  • View Affiliations

  • Published online on: October 1, 2005     https://doi.org/10.3892/ijmm.16.4.753
  • Pages: 753-758
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Abstract

Interferon-γ (IFN-γ) is considered one of the main inflammatory cytokines contributing to the generation of anemia of chronic disease (ACD). In this study, we used a previously described murine model for ACD based on sublethal cecal ligation and puncture (CLP) with ensuing protracted peritonitis. Within 2 weeks after CLP, a moderate normochromic anemia with low serum iron concentration and preserved iron stores develops, which is consistent with ACD. In order to determine whether IFN-γ contributes to the development of ACD in vivo, we neutralized IFN-γ after CLP shortly before and during the phase of most severe bone marrow depression in order to prevent anemia. Additionally, we studied IFN-γ receptor-deficient mice that underwent CLP. Two weeks after CLP, we determined the red blood cell count, hemoglobin concentration, hematocrit, serum iron concentration, and iron stores in spleens of wild-type mice, IFN-γ receptor-deficient mice, and mice after neutralization of IFN-γ. Neutralization of IFN-γ after CLP could not prevent mice from becoming anemic. Accordingly, IFN-γ receptor-deficient mice developed anemia to the same extent as wild-type mice. Serum iron concentration was lowered both in IFN-γ receptor-deficient and wild-type mice. Iron stores in untreated IFN-γ receptor-deficient mice were elevated compared to untreated wild-type mice. After CLP both IFN-γ receptor-deficient and wild-type mice had equally overloaded iron stores. Additional neutralization of TNF in IFN-γ receptor-deficient mice also did not attenuate CLP-induced anemia. Our results clearly demonstrate that neither IFN-γ alone nor in combination with TNF is a mediator of ACD in our model with transient anemia induced by protracted septic peritonitis.

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October 2005
Volume 16 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Schubert TE, Echtenacher B, Hofstädter F and Männel DN: Failure of interferon-γ and tumor necrosis factor in mediating anemia of chronic disease in a mouse model of protracted septic peritonitis. Int J Mol Med 16: 753-758, 2005
APA
Schubert, T.E., Echtenacher, B., Hofstädter, F., & Männel, D.N. (2005). Failure of interferon-γ and tumor necrosis factor in mediating anemia of chronic disease in a mouse model of protracted septic peritonitis. International Journal of Molecular Medicine, 16, 753-758. https://doi.org/10.3892/ijmm.16.4.753
MLA
Schubert, T. E., Echtenacher, B., Hofstädter, F., Männel, D. N."Failure of interferon-γ and tumor necrosis factor in mediating anemia of chronic disease in a mouse model of protracted septic peritonitis". International Journal of Molecular Medicine 16.4 (2005): 753-758.
Chicago
Schubert, T. E., Echtenacher, B., Hofstädter, F., Männel, D. N."Failure of interferon-γ and tumor necrosis factor in mediating anemia of chronic disease in a mouse model of protracted septic peritonitis". International Journal of Molecular Medicine 16, no. 4 (2005): 753-758. https://doi.org/10.3892/ijmm.16.4.753