Antiproliferative and apoptosis-inducing activity of Brucea javanica extract on human carcinoma cells

  • Authors:
    • Fung Yi Lau
    • Chung Hin Chui
    • Roberto Gambari
    • Stanton Hon Lung Kok
    • Kin Luen Kan
    • Gregory Yin Ming Cheng
    • Raymond Siu Ming Wong
    • Ivy Tuang Ngo Teo
    • Chor Hing Cheng
    • Thomas Shek Kwong Wan
    • Albert Sun Chi Chan
    • Johnny Cheuk On Tang
  • View Affiliations

  • Published online on: December 1, 2005     https://doi.org/10.3892/ijmm.16.6.1157
  • Pages: 1157-1162
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Abstract

We have recently demonstrated the antiproliferative and apoptotic activities of herbal traditional Chinese medicines, including the analomous fruit extract of Gleditsia sinensis, the fresh juice of Scutellaria barbata and the warmed water extract of Radix Sophorae Tonkinensis on a series of human carcinoma cells. Here, we further report the potential anti-cancer activity of the warmed water extract of Brucea javanica (BJE). Four cancer cell lines, including A549 non-small cell lung cancer, Hep3B hepatocellular carcinoma, MDA-MB231 breast cancer and SLMT-1 oesophageal squamous cell carcinoma, were incubated with BJE and strong apoptotic induction was observed under inverted microscopic investigation for all of the four cell lines tested. Using the MDA-MB231 breast cancer cell line as an experimental model, additional analyses supported the hypothesis that the mitochondrial membrane potential depolarization pathway was induced by BJE. The APO-1/Fas receptor death induction pathway was not activated under the influence of BJE, as studied by staining with Fas ligand and Fas receptor specific antibodies. Accordingly, only weak activation of caspase 8 was observed upon BJE treatment. On the other hand, caspase 3 activity was stimulated up to five-fold in BJE-treated cells compared to untreated controls. Oligonucleosomal DNA fragmentation formation was detected by labelling the nucleic acid ladders with TdT-mediated dUTP nick end labelling. Collectively, BJE-induced cancer cell death proceeds through a mitochondrial dependent pathway associated with caspase 3 activation.

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December 2005
Volume 16 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Lau, F.Y., Chui, C.H., Gambari, R., Kok, S.H., Kan, K.L., Cheng, G.Y. ... Tang, J.C. (2005). Antiproliferative and apoptosis-inducing activity of Brucea javanica extract on human carcinoma cells. International Journal of Molecular Medicine, 16, 1157-1162. https://doi.org/10.3892/ijmm.16.6.1157
MLA
Lau, F. Y., Chui, C. H., Gambari, R., Kok, S. H., Kan, K. L., Cheng, G. Y., Wong, R. S., Teo, I. T., Cheng, C. H., Wan, T. S., Chan, A. S., Tang, J. C."Antiproliferative and apoptosis-inducing activity of Brucea javanica extract on human carcinoma cells". International Journal of Molecular Medicine 16.6 (2005): 1157-1162.
Chicago
Lau, F. Y., Chui, C. H., Gambari, R., Kok, S. H., Kan, K. L., Cheng, G. Y., Wong, R. S., Teo, I. T., Cheng, C. H., Wan, T. S., Chan, A. S., Tang, J. C."Antiproliferative and apoptosis-inducing activity of Brucea javanica extract on human carcinoma cells". International Journal of Molecular Medicine 16, no. 6 (2005): 1157-1162. https://doi.org/10.3892/ijmm.16.6.1157