A proteomics approach to characterizing human submandibular gland cell lines by fluorescent two-dimensional differential in-gel electrophoresis

  • Authors:
    • Atsushi Kasamatsu
    • Katsuhiro Uzawa
    • Dai Nakashima
    • Yukinao Kouzu
    • Yosuke Endo
    • Hirofumi Koike
    • Hidetaka Yokoe
    • Koji Harada
    • Mitsunobu Sato
    • Hideki Tanzawa
  • View Affiliations

  • Published online on: February 1, 2006     https://doi.org/10.3892/ijmm.17.2.253
  • Pages: 253-260
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Abstract

The human salivary glands have a variety of histologic features such as intercalated duct cells, myoepithelial cells and acinar cells. A neoplastic human salivary intercalated duct cell line (HSG) and its derivatives, HSG with a myoepithelial cell phenotype (HSG-AZA1) and HSG with an acinar cell phenotype (HSG-AZA3) induced by 5-aza-2'-dC treatment of HSG cells, have been reported. To identify characterization of intercalated duct cells, myoepithelial cells and acinar cells in the salivary gland, we selected HSG, HSG-AZA1 and HSG-AZA3 cell lines to perform two-dimensional electrophoresis analysis. We used a fluorescent two-dimensional differential in-gel electrophoresis (2-D-DIGE) for comparative proteomics, which improved the reproducibility and reliability of differential protein expression analysis between the samples. Furthermore, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) peptide mass fingerprinting (PMF) was used to identify the proteins. These methods were combined to approach the protein profiles associated with characterization between HSG, HSG-AZA1 and HSG-AZA3 cells. Using these strategies, we identified seven HSG associated proteins, such as actin-β, hydrocephalus inducing protein, L-plastin, KIAA0657 protein, septin 6 isoform A, lamin A/C isoform 2 and superoxide dismutase 2, three HSG-AZA1 associated proteins such as ubiquitin carboxyl-terminal esterase L1, myosin light chain 2 and muscle creatine kinase, and two HSG-AZA3 associated proteins, microtubule-associated protein 6 and Annexin A3. These results suggest that the proteins are associated with characterization of the salivary gland.

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February 2006
Volume 17 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kasamatsu A, Uzawa K, Nakashima D, Kouzu Y, Endo Y, Koike H, Yokoe H, Harada K, Sato M, Tanzawa H, Tanzawa H, et al: A proteomics approach to characterizing human submandibular gland cell lines by fluorescent two-dimensional differential in-gel electrophoresis. Int J Mol Med 17: 253-260, 2006.
APA
Kasamatsu, A., Uzawa, K., Nakashima, D., Kouzu, Y., Endo, Y., Koike, H. ... Tanzawa, H. (2006). A proteomics approach to characterizing human submandibular gland cell lines by fluorescent two-dimensional differential in-gel electrophoresis. International Journal of Molecular Medicine, 17, 253-260. https://doi.org/10.3892/ijmm.17.2.253
MLA
Kasamatsu, A., Uzawa, K., Nakashima, D., Kouzu, Y., Endo, Y., Koike, H., Yokoe, H., Harada, K., Sato, M., Tanzawa, H."A proteomics approach to characterizing human submandibular gland cell lines by fluorescent two-dimensional differential in-gel electrophoresis". International Journal of Molecular Medicine 17.2 (2006): 253-260.
Chicago
Kasamatsu, A., Uzawa, K., Nakashima, D., Kouzu, Y., Endo, Y., Koike, H., Yokoe, H., Harada, K., Sato, M., Tanzawa, H."A proteomics approach to characterizing human submandibular gland cell lines by fluorescent two-dimensional differential in-gel electrophoresis". International Journal of Molecular Medicine 17, no. 2 (2006): 253-260. https://doi.org/10.3892/ijmm.17.2.253