Molecular characterization of the zinc finger transcription factor, Osterix

Hatta,Mitsutoki; Yoshimura,Yoshitaka; Deyama,Yoshiaki; Fukamizu,Akiyoshi; Suzuki,Kuniaki

doi:10.3892/ijmm.17.3.425

March 2006 Volume 17 Issue 3

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March 2006 Volume 17 Issue 3

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Article

Molecular characterization of the zinc finger transcription factor, Osterix

Authors:
- Mitsutoki Hatta
- Yoshitaka Yoshimura
- Yoshiaki Deyama
- Akiyoshi Fukamizu
- Kuniaki Suzuki
View Affiliations / Copyright

Affiliations: Department of Oral Pathobiological Science, Hokkaido University, Sapporo 060-8586, Japan
Pages: 425-430
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Published online on: March 1, 2006

https://doi.org/10.3892/ijmm.17.3.425
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Abstract

Osterix was identified as a transcription factor expressing, in osteoblasts, required for bone formation. However, the molecular mechanisms of the gene regulation by Osterix remain elusive. In this study, we examined the transactivation property of Osterix by using the Gal4 fusion system reporter assay. We identified the transactivation domain of Osterix, which contains high proline and glycine residues and has an activation property in mammalian and yeast cells. The GST-pull down analysis revealed that the basal transcription factor, TF-IIB, but not TBP, binds to the transactivation domain. Furthermore, we found that Osterix interacts with chromatin remodeling factor, Brg-1, through its C-terminal zinc finger domain in vivo and in vitro. These findings suggest that Osterix possesses functional domains which associate with transcription mediated factors and functions as a transcriptional activator in the nucleus.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Molecular characterization of the zinc finger transcription factor, Osterix

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Abstract

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