Enhanced cell-substratum adhesion of E-cadherin-expressing cells is mediated by activation of the small GTPase protein, Rac1

Wang,Yuqing; Ohkubo,Tadashi; Tsubouchi,Hirohito; Ozawa,Masayuki

doi:10.3892/ijmm.17.4.637

April 2006 Volume 17 Issue 4

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April 2006 Volume 17 Issue 4

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Article

Enhanced cell-substratum adhesion of E-cadherin-expressing cells is mediated by activation of the small GTPase protein, Rac1

Authors:
- Yuqing Wang
- Tadashi Ohkubo
- Hirohito Tsubouchi
- Masayuki Ozawa
View Affiliations / Copyright

Affiliations: Departments of Biochemistry and Molecular Biology, Internal Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan
Pages: 637-642
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Published online on: April 1, 2006

https://doi.org/10.3892/ijmm.17.4.637
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Abstract

L cell transfectants stably expressing E-cadherin demonstrate a remarkable increase in adherence to extracellular matrix proteins, such as type IV collagen and fibronectin. This enhanced adhesion is mediated by integrin-type cell surface receptors, as assessed by inhibition with anti-receptor antibodies. Both the rate and efficiency of adhesion were enhanced 4- to 5-fold. In contrast, non-specific adhesion processes, such as cell attachment to polylysine-coated substrata, are unaffected by E-cadherin expression. Thus, integrin-mediated but not non-specific adhesion is modulated by E-cadherin expression. L cells expressing mutant E-cadherin molecules either lacking the cytoplasmic domain or bearing an amino acid substitution in the Ca2+-binding motif did not exhibit enhanced adhesion. The amount of collagen receptor, the α1 and β1 integrin, did not change following expression of E-cadherin. Pull-down assays with the Cdc42/Rac interactive binding (CRIB) domain of the Rac effector, p21-activated kinase, revealed increased Rac-GTP levels in cells expressing wild-type E-cadherin. These results suggest that the activation of Rac is involved in the enhancement of integrin-mediated adhesion induced by E-cadherin expression.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Enhanced cell-substratum adhesion of E-cadherin-expressing cells is mediated by activation of the small GTPase protein, Rac1

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