Effect of cadmium-induced oxidative stress on antioxidative enzymes in mitochondria and cytoplasm of CRL-1439 rat liver cells

  • Authors:
    • Lekan M. Latinwo
    • Veera L. Badisa
    • Christopher O. Ikediobi
    • Caroline O. Odewumi
    • Ayuk-Takem T. Lambert
    • Ramesh B. Badisa
  • View Affiliations

  • Published online on: September 1, 2006     https://doi.org/10.3892/ijmm.18.3.477
  • Pages: 477-481
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Cadmium affects human health through occupational and environmental exposure. In this report, we present the response of mitochondrial and cytoplasmic antioxidant enzymes of CRL-1439 cells exposed to different concentrations (0-150 µM) of CdCl2 for 24 h at 37°C. Exposure of liver cells to 50 µM CdCl2 increased mitochondrial catalase and glutathione reductase (GR) activities more than the cytoplasmic enzymes. Although the mitochondrial selenium-dependent glutathione peroxidase (Se-GPx) showed less enzymatic activity than the cytoplasmic enzyme, the mitochondrial selenium-independent glutathione peroxidase (non-Se-GPx) showed a slight increase in activity over its cytoplasmic counterpart compared to untreated controls. With 100 µM CdCl2, catalase maintained an increase in specific activity in mitochondria over the cytoplasmic enzyme compared to the controls. The level of GR was higher in the cytoplasm than in the mitochondria. However, the activity of Se-GPx and non-Se-GPx decreased slightly in the mitochondria compared to their cytoplasmic counterparts. Exposure of cells to 150 µM CdCl2 decreased all antioxidant enzyme activities compared to the 100 µM CdCl2-treated samples due to toxic effect. Each antioxidant enzyme exhibited its own pattern of activation or inhibition upon exposure to different concentrations of cadmium, with more oxidative stress observed in the mitochondria.

Related Articles

Journal Cover

September 2006
Volume 18 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Latinwo LM, Badisa VL, Ikediobi CO, Odewumi CO, Lambert AT and Badisa RB: Effect of cadmium-induced oxidative stress on antioxidative enzymes in mitochondria and cytoplasm of CRL-1439 rat liver cells. Int J Mol Med 18: 477-481, 2006
APA
Latinwo, L.M., Badisa, V.L., Ikediobi, C.O., Odewumi, C.O., Lambert, A.T., & Badisa, R.B. (2006). Effect of cadmium-induced oxidative stress on antioxidative enzymes in mitochondria and cytoplasm of CRL-1439 rat liver cells. International Journal of Molecular Medicine, 18, 477-481. https://doi.org/10.3892/ijmm.18.3.477
MLA
Latinwo, L. M., Badisa, V. L., Ikediobi, C. O., Odewumi, C. O., Lambert, A. T., Badisa, R. B."Effect of cadmium-induced oxidative stress on antioxidative enzymes in mitochondria and cytoplasm of CRL-1439 rat liver cells". International Journal of Molecular Medicine 18.3 (2006): 477-481.
Chicago
Latinwo, L. M., Badisa, V. L., Ikediobi, C. O., Odewumi, C. O., Lambert, A. T., Badisa, R. B."Effect of cadmium-induced oxidative stress on antioxidative enzymes in mitochondria and cytoplasm of CRL-1439 rat liver cells". International Journal of Molecular Medicine 18, no. 3 (2006): 477-481. https://doi.org/10.3892/ijmm.18.3.477