Effects of lysine to arginine mutations in HIV-1 Vif on its expression and viral infectivity

  • Authors:
    • Boonruang Khamsri
    • Mikako Fujita
    • Kazuya Kamada
    • Ahmad Piroozmand
    • Tomoki Yamashita
    • Tsuneo Uchiyama
    • Akio Adachi
  • View Affiliations

  • Published online on: October 1, 2006     https://doi.org/10.3892/ijmm.18.4.679
  • Pages: 679-683
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We previously demonstrated that the expression in cells of human immunodeficiency virus type 1 (HIV-1) Vif is maintained at low level by proteasome-degradation. We examined the contribution of 16 lysines present in Vif (NL432 clone), which is composed of 192 amino acids (aa), to its expression within cells and to viral infectivity for non-permissive cells. To this end, various lysine-arginine mutations were introduced into wild-type (wt) Vif, and the mutational effects were monitored by transfection experiments. When all the lysines were changed to arginines, the mutant Vif was expressed in cells at much higher level than wt and was much more stable. Both N-terminal (aa nos. 34 and 36) and C-terminal (aa nos. 179 and 181) lysines were found to be almost sufficient for wt property. Different from this observation, one of the lysines at aa nos. 22 and 26 was demonstrated to be essential for the virus to grow in non-permissive cells. Our results showed that there is no clear co-relationship between the expression level of HIV-1 Vif and viral infectivity.

Related Articles

Journal Cover

October 2006
Volume 18 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Khamsri B, Fujita M, Kamada K, Piroozmand A, Yamashita T, Uchiyama T and Adachi A: Effects of lysine to arginine mutations in HIV-1 Vif on its expression and viral infectivity. Int J Mol Med 18: 679-683, 2006
APA
Khamsri, B., Fujita, M., Kamada, K., Piroozmand, A., Yamashita, T., Uchiyama, T., & Adachi, A. (2006). Effects of lysine to arginine mutations in HIV-1 Vif on its expression and viral infectivity. International Journal of Molecular Medicine, 18, 679-683. https://doi.org/10.3892/ijmm.18.4.679
MLA
Khamsri, B., Fujita, M., Kamada, K., Piroozmand, A., Yamashita, T., Uchiyama, T., Adachi, A."Effects of lysine to arginine mutations in HIV-1 Vif on its expression and viral infectivity". International Journal of Molecular Medicine 18.4 (2006): 679-683.
Chicago
Khamsri, B., Fujita, M., Kamada, K., Piroozmand, A., Yamashita, T., Uchiyama, T., Adachi, A."Effects of lysine to arginine mutations in HIV-1 Vif on its expression and viral infectivity". International Journal of Molecular Medicine 18, no. 4 (2006): 679-683. https://doi.org/10.3892/ijmm.18.4.679