The effects of macrophage migratory inhibitory factor on acute-phase protein production in primary human hepatocytes

  • Authors:
    • Nicholas M. Wheelhouse
    • Nabil Dowidar
    • Cornelis H.C. Dejong
    • O. James Garden
    • James J. Powell
    • Matthew D. Barber
    • Kathryn Sangster
    • Jean P. Maingay
    • James A. Ross
  • View Affiliations

  • Published online on: November 1, 2006     https://doi.org/10.3892/ijmm.18.5.957
  • Pages: 957-961
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Abstract

Macrophage inhibitory factor (MIF) is a pituitary peptide released during the physiological stress response, a T-cell product secreted during the antigen-specific response and a pro-inflammatory macrophage cytokine secreted after LPS stimulation. It has become apparent that MIF is central to the regulation of the inflammatory response and is implicated in the pathogenesis of a variety of acute and chronic inflammatory conditions. This is, at least in part, due to the apparent counter-regulation of the anti-inflammatory actions of glucocorticoids, including the reversal of glucocorticoid-mediated IL-6 release inhibition. This study examines the effect of recombinant MIF on regulation of the acute phase response in isolated human hepatocytes. MIF alone increased C-reactive protein (CRP) release in a dose-dependent manner ≤0.1 ng/ml after which the effects of MIF were attenuated. In combination with IL-6 both CRP and and α-1-antichymotrypsin (ACT) release were increased above levels found with either IL-6 or MIF treatment alone. Dexamethasone attenuated the effects of MIF upon CRP production but increased the MIF stimulated release of ACT. The study demonstrates that the effects of MIF upon the acute phase response are complex and can differentially modulate the production of acute phase proteins depending on the presence of other factors.

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November 2006
Volume 18 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wheelhouse NM, Dowidar N, Dejong CH, Garden OJ, Powell JJ, Barber MD, Sangster K, Maingay JP and Ross JA: The effects of macrophage migratory inhibitory factor on acute-phase protein production in primary human hepatocytes. Int J Mol Med 18: 957-961, 2006.
APA
Wheelhouse, N.M., Dowidar, N., Dejong, C.H., Garden, O.J., Powell, J.J., Barber, M.D. ... Ross, J.A. (2006). The effects of macrophage migratory inhibitory factor on acute-phase protein production in primary human hepatocytes. International Journal of Molecular Medicine, 18, 957-961. https://doi.org/10.3892/ijmm.18.5.957
MLA
Wheelhouse, N. M., Dowidar, N., Dejong, C. H., Garden, O. J., Powell, J. J., Barber, M. D., Sangster, K., Maingay, J. P., Ross, J. A."The effects of macrophage migratory inhibitory factor on acute-phase protein production in primary human hepatocytes". International Journal of Molecular Medicine 18.5 (2006): 957-961.
Chicago
Wheelhouse, N. M., Dowidar, N., Dejong, C. H., Garden, O. J., Powell, J. J., Barber, M. D., Sangster, K., Maingay, J. P., Ross, J. A."The effects of macrophage migratory inhibitory factor on acute-phase protein production in primary human hepatocytes". International Journal of Molecular Medicine 18, no. 5 (2006): 957-961. https://doi.org/10.3892/ijmm.18.5.957