CD105(+) cells from Wharton's jelly show in vitro and in vivo myogenic differentiative potential

  • Authors:
    • Maria Teresa Conconi
    • Patrizia Burra
    • Rosa Di Liddo
    • Chiara Calore
    • Michela Turetta
    • Silvia Bellini
    • Patrizio Bo
    • Gastone G. Nussdorfer
    • Pier Paolo Parnigotto
  • View Affiliations

  • Published online on: December 1, 2006     https://doi.org/10.3892/ijmm.18.6.1089
  • Pages: 1089-1096
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Abstract

Embryo-derived tissues, such as umbilical cord (UC), can represent attractive sources of mesenchymal stem cells because their use is not related to any ethical issue. Abundant experimental evidence has already shown that Wharton's jelly contains cells able to differentiate in vitro into adipocytes, chondrocytes, osteocytes and neurons. Human UCs were obtained from term caesarean deliveries and processed within 24 h. Cells derived from the Wharton's jelly expressing mesenchymal markers, such as CD105, but not KDR and CD31 antigens, have been selected by positive and negative immunoseparation. These cells were characterized by an elongated shape and a good proliferation rate. Moreover, they were, at least in part, of fetal origin, as demonstrated by the expression of Sry mRNA. The expression of Myf5 and MyoD was detected after 7 and 11 days of in vitro myogenic induction, respectively. At two weeks from cell injection in the tibialis anterior muscle, previously damaged with bupivacaine, skeletal muscle appeared completely repaired and transplanted cells were present in the muscle for two weeks and differentiated into skeletal muscle cells, as demonstrated by the co-localization of HLA 1 and sarcomeric tropomyosine antigens. These observations provide the first demonstration that CD105(+)/CD31(−)/KDR(−) cells are able not only to differentiate in vivo towards the myogenic lineage, but also to contribute to the muscle regenerative process.

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December 2006
Volume 18 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Conconi MT, Burra P, Di Liddo R, Calore C, Turetta M, Bellini S, Bo P, Nussdorfer GG and Parnigotto PP: CD105(+) cells from Wharton's jelly show in vitro and in vivo myogenic differentiative potential. Int J Mol Med 18: 1089-1096, 2006
APA
Conconi, M.T., Burra, P., Di Liddo, R., Calore, C., Turetta, M., Bellini, S. ... Parnigotto, P.P. (2006). CD105(+) cells from Wharton's jelly show in vitro and in vivo myogenic differentiative potential. International Journal of Molecular Medicine, 18, 1089-1096. https://doi.org/10.3892/ijmm.18.6.1089
MLA
Conconi, M. T., Burra, P., Di Liddo, R., Calore, C., Turetta, M., Bellini, S., Bo, P., Nussdorfer, G. G., Parnigotto, P. P."CD105(+) cells from Wharton's jelly show in vitro and in vivo myogenic differentiative potential". International Journal of Molecular Medicine 18.6 (2006): 1089-1096.
Chicago
Conconi, M. T., Burra, P., Di Liddo, R., Calore, C., Turetta, M., Bellini, S., Bo, P., Nussdorfer, G. G., Parnigotto, P. P."CD105(+) cells from Wharton's jelly show in vitro and in vivo myogenic differentiative potential". International Journal of Molecular Medicine 18, no. 6 (2006): 1089-1096. https://doi.org/10.3892/ijmm.18.6.1089