Cloning and characterization of a novel sperm tail protein, NYD-SP28

  • Authors:
    • Ying Zheng
    • Jingjing Zhang
    • Lei Wang
    • Zuomin Zhou
    • Min Xu
    • Jianmin Li
    • Jia-hao Sha
  • View Affiliations

  • Published online on: Friday, December 1, 2006
  • Pages: 1119-1125 DOI: 10.3892/ijmm.18.6.1119

Abstract

In this study, a gene coding a novel human sperm tail protein named NYD-SP28 was cloned and characterized using a complementary DNA (cDNA) microarray. Its expression was 3.5 times higher in human testis than in fetal testis, and very high in human spermatozoa. The full length of NYD-SP28 cDNA was 1798 bp and encoded a 484-amino-acid protein. Motif analysis revealed that the protein contained a cluster of phosphorylation sites, N-glycosylation sites and N-myristoylation sites. Immunohistochemical analysis of normal human testes showed that NYD-SP28 was expressed in the cytoplasm of spermatogenic cells but not in interstitial cells. The EGFP-NYD-SP28 fusion protein was also localized in the cytoplasm of transfected 7721 cells. In human spermatozoa, NYD-SP28 immunoreactivity was detected in entire sperm tail. Using the two-dimensional (2-D) gel electrophoresis and immunoblotting technique, NYD-SP28 was found to be post-translationally modified during sperm capacitation. In conclusion, these results suggest that NYD-SP28 is a new human sperm tail protein and might play an important role during sperm capacitation.

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December 2006
Volume 18 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2015 Impact Factor: 2.348
Ranked #22/123 Medicine Research and Experimental
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APA
Zheng, Y., Zhang, J., Wang, L., Zhou, Z., Xu, M., Li, J., & Sha, J. (2006). Cloning and characterization of a novel sperm tail protein, NYD-SP28. International Journal of Molecular Medicine, 18, 1119-1125. http://dx.doi.org/10.3892/ijmm.18.6.1119
MLA
Zheng, Y., Zhang, J., Wang, L., Zhou, Z., Xu, M., Li, J., Sha, J."Cloning and characterization of a novel sperm tail protein, NYD-SP28". International Journal of Molecular Medicine 18.6 (2006): 1119-1125.
Chicago
Zheng, Y., Zhang, J., Wang, L., Zhou, Z., Xu, M., Li, J., Sha, J."Cloning and characterization of a novel sperm tail protein, NYD-SP28". International Journal of Molecular Medicine 18, no. 6 (2006): 1119-1125. http://dx.doi.org/10.3892/ijmm.18.6.1119