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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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January 2007 Volume 19 Issue 1

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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January 2007 Volume 19 Issue 1

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Article

A three-step purification method of large quantities of human recombinant α endothelial cellular growth factor for clinical use

  • Authors:
    • Alexander Sauter
    • Katherine L. Lambert
    • Ann-Katrin Rupf
    • Bernd-Ulrich Von Specht
    • Karl Hörmann
    • Ramin Naim

  • View Affiliations / Copyright

    Affiliations: Univ.-HNO-Klinik, Universitätsklinikum Mannheim, D-68135 Mannheim, Germany. alexander.sauter@hno.ma.uni-heidelberg.de
  • Pages: 97-103
    |
    Published online on: January 1, 2007
       https://doi.org/10.3892/ijmm.19.1.97
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Abstract

The endothelial cellular growth factor α-ECGF is a candidate drug for the induction of therapeutic neoangiogenesis. Its use in extensive experimental and clinical trials is hampered by the fact that currently published purification procedures allow only small yields, and the absence of pyrogenic impurities is not demonstrated. The rh α-ECGF was expressed in E. coli. Isolation of rh α-ECGF from E. coli lysates to apparent homogenicity was achieved by a three step purification procedure involving ionic exchange, heparin-sepharose and polymyxin B chromatography. By this method, 200 mg of rh α-ECGF was purified from 15 g wet weight E. coli bacteria. The isolated protein of 18 kDa appeared as a single band after SDS gel electrophoresis and subsequent silver-staining. The biological activity was expressed in the chorion-allantois-membrane assay and in the 3H-thymidine proliferation in baby hamster kidney cells. Drug trials with rabbits revealed no increase in body temperature after intravenous injections with 1 mg rh-ECGF.

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Copy and paste a formatted citation
Spandidos Publications style
Sauter A, Lambert KL, Rupf A, Von Specht B, Hörmann K and Naim R: A three-step purification method of large quantities of human recombinant α endothelial cellular growth factor for clinical use. Int J Mol Med 19: 97-103, 2007.
APA
Sauter, A., Lambert, K.L., Rupf, A., Von Specht, B., Hörmann, K., & Naim, R. (2007). A three-step purification method of large quantities of human recombinant α endothelial cellular growth factor for clinical use. International Journal of Molecular Medicine, 19, 97-103. https://doi.org/10.3892/ijmm.19.1.97
MLA
Sauter, A., Lambert, K. L., Rupf, A., Von Specht, B., Hörmann, K., Naim, R."A three-step purification method of large quantities of human recombinant α endothelial cellular growth factor for clinical use". International Journal of Molecular Medicine 19.1 (2007): 97-103.
Chicago
Sauter, A., Lambert, K. L., Rupf, A., Von Specht, B., Hörmann, K., Naim, R."A three-step purification method of large quantities of human recombinant α endothelial cellular growth factor for clinical use". International Journal of Molecular Medicine 19, no. 1 (2007): 97-103. https://doi.org/10.3892/ijmm.19.1.97
Copy and paste a formatted citation
x
Spandidos Publications style
Sauter A, Lambert KL, Rupf A, Von Specht B, Hörmann K and Naim R: A three-step purification method of large quantities of human recombinant α endothelial cellular growth factor for clinical use. Int J Mol Med 19: 97-103, 2007.
APA
Sauter, A., Lambert, K.L., Rupf, A., Von Specht, B., Hörmann, K., & Naim, R. (2007). A three-step purification method of large quantities of human recombinant α endothelial cellular growth factor for clinical use. International Journal of Molecular Medicine, 19, 97-103. https://doi.org/10.3892/ijmm.19.1.97
MLA
Sauter, A., Lambert, K. L., Rupf, A., Von Specht, B., Hörmann, K., Naim, R."A three-step purification method of large quantities of human recombinant α endothelial cellular growth factor for clinical use". International Journal of Molecular Medicine 19.1 (2007): 97-103.
Chicago
Sauter, A., Lambert, K. L., Rupf, A., Von Specht, B., Hörmann, K., Naim, R."A three-step purification method of large quantities of human recombinant α endothelial cellular growth factor for clinical use". International Journal of Molecular Medicine 19, no. 1 (2007): 97-103. https://doi.org/10.3892/ijmm.19.1.97
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