A three-step purification method of large quantities of human recombinant α endothelial cellular growth factor for clinical use

  • Authors:
    • Alexander Sauter
    • Katherine L. Lambert
    • Ann-Katrin Rupf
    • Bernd-Ulrich Von Specht
    • Karl Hörmann
    • Ramin Naim
  • View Affiliations

  • Published online on: January 1, 2007     https://doi.org/10.3892/ijmm.19.1.97
  • Pages: 97-103
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Abstract

The endothelial cellular growth factor α-ECGF is a candidate drug for the induction of therapeutic neoangiogenesis. Its use in extensive experimental and clinical trials is hampered by the fact that currently published purification procedures allow only small yields, and the absence of pyrogenic impurities is not demonstrated. The rh α-ECGF was expressed in E. coli. Isolation of rh α-ECGF from E. coli lysates to apparent homogenicity was achieved by a three step purification procedure involving ionic exchange, heparin-sepharose and polymyxin B chromatography. By this method, 200 mg of rh α-ECGF was purified from 15 g wet weight E. coli bacteria. The isolated protein of 18 kDa appeared as a single band after SDS gel electrophoresis and subsequent silver-staining. The biological activity was expressed in the chorion-allantois-membrane assay and in the 3H-thymidine proliferation in baby hamster kidney cells. Drug trials with rabbits revealed no increase in body temperature after intravenous injections with 1 mg rh-ECGF.

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January 2007
Volume 19 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sauter A, Lambert KL, Rupf A, Von Specht B, Hörmann K and Naim R: A three-step purification method of large quantities of human recombinant α endothelial cellular growth factor for clinical use. Int J Mol Med 19: 97-103, 2007
APA
Sauter, A., Lambert, K.L., Rupf, A., Von Specht, B., Hörmann, K., & Naim, R. (2007). A three-step purification method of large quantities of human recombinant α endothelial cellular growth factor for clinical use. International Journal of Molecular Medicine, 19, 97-103. https://doi.org/10.3892/ijmm.19.1.97
MLA
Sauter, A., Lambert, K. L., Rupf, A., Von Specht, B., Hörmann, K., Naim, R."A three-step purification method of large quantities of human recombinant α endothelial cellular growth factor for clinical use". International Journal of Molecular Medicine 19.1 (2007): 97-103.
Chicago
Sauter, A., Lambert, K. L., Rupf, A., Von Specht, B., Hörmann, K., Naim, R."A three-step purification method of large quantities of human recombinant α endothelial cellular growth factor for clinical use". International Journal of Molecular Medicine 19, no. 1 (2007): 97-103. https://doi.org/10.3892/ijmm.19.1.97