Toll-like receptor 3 ligand-induced antiviral response in mouse osteoblastic cells

  • Authors:
    • Kimiya Nakamura
    • Yoshiaki Deyama
    • Yoshitaka Yoshimura
    • Kuniaki Suzuki
    • Manabu Morita
  • View Affiliations

  • Published online on: May 1, 2007     https://doi.org/10.3892/ijmm.19.5.771
  • Pages: 771-775
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Abstract

Double-stranded RNA (dsRNA) and its mimic, polyinosinic acid:polycytidylic acid [poly(I):poly(C)], are recognized by toll-like receptor 3 (TLR3) that induces the production of IFN-β in many cell types. In the present study, we investigated the effects of poly(I):poly(C) on mouse osteoblastic MC3T3-E1 (E1) cells. Poly(I):poly(C) markedly increased IFN-β mRNA level in a dose-dependent manner. The increase in the IFN-β mRNA level was apparent as early as 1 h after adding poly(I):poly(C) to the culture and peaked at 12 h. Stimulation with poly(I):poly(C) enhanced the expression of CXCL10 mRNA and TLR3 in E1 cells. Moreover, poly(I):poly(C) induced tyrosine phosphorylation of the transcription factor STAT1 in E1 cells. An anti-IFN-β neutralizing antibody partially inhibited poly(I):poly(C)-induced CXCL10 mRNA, TLR3 mRNA and STAT1 phosphorylation. These results indicate that osteoblasts secrete IFN-β in response to viral infection and that endogenous IFN-β induces both CXCL10 and TLR3 production via an IFN-α/β receptor-STAT1 pathway. It is suggested that osteoblasts are involved in host defense as well as bone metabolism.

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May 2007
Volume 19 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Nakamura K, Deyama Y, Yoshimura Y, Suzuki K and Morita M: Toll-like receptor 3 ligand-induced antiviral response in mouse osteoblastic cells. Int J Mol Med 19: 771-775, 2007.
APA
Nakamura, K., Deyama, Y., Yoshimura, Y., Suzuki, K., & Morita, M. (2007). Toll-like receptor 3 ligand-induced antiviral response in mouse osteoblastic cells. International Journal of Molecular Medicine, 19, 771-775. https://doi.org/10.3892/ijmm.19.5.771
MLA
Nakamura, K., Deyama, Y., Yoshimura, Y., Suzuki, K., Morita, M."Toll-like receptor 3 ligand-induced antiviral response in mouse osteoblastic cells". International Journal of Molecular Medicine 19.5 (2007): 771-775.
Chicago
Nakamura, K., Deyama, Y., Yoshimura, Y., Suzuki, K., Morita, M."Toll-like receptor 3 ligand-induced antiviral response in mouse osteoblastic cells". International Journal of Molecular Medicine 19, no. 5 (2007): 771-775. https://doi.org/10.3892/ijmm.19.5.771