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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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Aug 1998 Volume 2 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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Aug 1998 Volume 2 Issue 2

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Article

In situ localization of paclitaxel binding structures: Labeling with a paclitaxel fluorescent analogue.

  • Authors:
    • C Bicamumpaka
    • M Page

  • View Affiliations / Copyright

    Affiliations: Department of Medical Biology, Faculty of Medicine, Laval University, Sainte Foy, Quebec G1K 7P4, Canada.
  • Pages: 161-326
    |
    Published online on: August 1, 1998
       https://doi.org/10.3892/ijmm.2.2.161
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Abstract

Microtubules are a major component of cell cytoskeleton. Microtubules constitute the cellular target of paclitaxel. The interaction of paclitaxel with microtubules causes an increase in tubulin polymerization and microtubules stabilization, leading to a G2/M phase cell cycle arrest and cell death by apoptosis. Three paclitaxel fluorescent analogues were prepared by introducing fluorescein or BODIPY moiety onto the 2' of the 7-carbon. These were then used to study the interaction of paclitaxel with its cellular binding site and the microtubule network was visualized directly by fluorescence microscopy. Free paclitaxel was able to inhibit 7-FITC paclitaxel binding, demonstrating that both products bind to the same site and possess similar biological properties. Using the carbon 7 derivatives, microtubules were labeled as cytoplasmic fibers extending centripedly. A few other cellular components such as nuclear membrane, nucleoli and some other cytoplasmic structures were also labeled. The labeling intensity was reduced by preincubation with free paclitaxel. The interaction of paclitaxel with microtubules was also investigated using flow cytometry. No binding of the 2'C derivative was detected, confirming that a free 2'C is a pre-requisite for paclitaxel microtubules interaction.

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Copy and paste a formatted citation
Spandidos Publications style
Bicamumpaka C and Page M: In situ localization of paclitaxel binding structures: Labeling with a paclitaxel fluorescent analogue.. Int J Mol Med 2: 161-326, 1998.
APA
Bicamumpaka, C., & Page, M. (1998). In situ localization of paclitaxel binding structures: Labeling with a paclitaxel fluorescent analogue.. International Journal of Molecular Medicine, 2, 161-326. https://doi.org/10.3892/ijmm.2.2.161
MLA
Bicamumpaka, C., Page, M."In situ localization of paclitaxel binding structures: Labeling with a paclitaxel fluorescent analogue.". International Journal of Molecular Medicine 2.2 (1998): 161-326.
Chicago
Bicamumpaka, C., Page, M."In situ localization of paclitaxel binding structures: Labeling with a paclitaxel fluorescent analogue.". International Journal of Molecular Medicine 2, no. 2 (1998): 161-326. https://doi.org/10.3892/ijmm.2.2.161
Copy and paste a formatted citation
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Spandidos Publications style
Bicamumpaka C and Page M: In situ localization of paclitaxel binding structures: Labeling with a paclitaxel fluorescent analogue.. Int J Mol Med 2: 161-326, 1998.
APA
Bicamumpaka, C., & Page, M. (1998). In situ localization of paclitaxel binding structures: Labeling with a paclitaxel fluorescent analogue.. International Journal of Molecular Medicine, 2, 161-326. https://doi.org/10.3892/ijmm.2.2.161
MLA
Bicamumpaka, C., Page, M."In situ localization of paclitaxel binding structures: Labeling with a paclitaxel fluorescent analogue.". International Journal of Molecular Medicine 2.2 (1998): 161-326.
Chicago
Bicamumpaka, C., Page, M."In situ localization of paclitaxel binding structures: Labeling with a paclitaxel fluorescent analogue.". International Journal of Molecular Medicine 2, no. 2 (1998): 161-326. https://doi.org/10.3892/ijmm.2.2.161
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