A study on the relationship between antisense EGFR cDNA fragments and nuclear matrix proteins in glioblastoma cells.

  • Authors:
    • Z H Wang
    • G H Yam
    • X X Tian
    • H K Ng
    • S B Chew-Cheng
    • M X Ding
    • E C Chew
  • View Affiliations

  • Published online on: October 1, 1998     https://doi.org/10.3892/ijmm.2.4.417
  • Pages: 417-438
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The association of antisense epidermal growth factor receptor (EGFR) cDNA fragments with nuclear matrix from EGFR-antisense transfected glioblastoma cell lines U343 and U87 was investigated. A 1015 bp DNA fragment (primer I-II) was amplified in both genomic DNA and nuclear matrix-associated DNA (NM DNA) from EGFR-antisense transfected glioblastoma cell lines U343E and U87E. Two different DNA fragments (940 bp and 110 bp) were amplified by primer I-III in both genomic DNA and NM DNA of U343E, while one 110 bp PCR product was shown with the same primer in both genomic DNA and NM DNA of U87E only. After EGFR-antisense transfection, the binding property of the 110 bp DNA fragment (primer IV-V) to nuclear matrix was not affected. Southwestern blotting demonstrated the presence of antisense EGFR cDNA binding nuclear matrix proteins. Our findings demonstrate that not only EGFR DNA is associated with nuclear matrix, but the transfected antisense EGFR cDNA also binds to nuclear matrix proteins. The nuclear matrix is most likely involved in the replication and transcription of antisense EGFR cDNA or hybridisation with sense mRNA in vitro.

Related Articles

Journal Cover

Oct 1998
Volume 2 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Yam G, Tian X, Ng H, Chew-Cheng S, Ding M and Chew E: A study on the relationship between antisense EGFR cDNA fragments and nuclear matrix proteins in glioblastoma cells.. Int J Mol Med 2: 417-438, 1998
APA
Wang, Z., Yam, G., Tian, X., Ng, H., Chew-Cheng, S., Ding, M., & Chew, E. (1998). A study on the relationship between antisense EGFR cDNA fragments and nuclear matrix proteins in glioblastoma cells.. International Journal of Molecular Medicine, 2, 417-438. https://doi.org/10.3892/ijmm.2.4.417
MLA
Wang, Z., Yam, G., Tian, X., Ng, H., Chew-Cheng, S., Ding, M., Chew, E."A study on the relationship between antisense EGFR cDNA fragments and nuclear matrix proteins in glioblastoma cells.". International Journal of Molecular Medicine 2.4 (1998): 417-438.
Chicago
Wang, Z., Yam, G., Tian, X., Ng, H., Chew-Cheng, S., Ding, M., Chew, E."A study on the relationship between antisense EGFR cDNA fragments and nuclear matrix proteins in glioblastoma cells.". International Journal of Molecular Medicine 2, no. 4 (1998): 417-438. https://doi.org/10.3892/ijmm.2.4.417