Quantification of Ggamma- and Agamma-globins by electrospray ionisation mass spectrometry.

Ofori-Acquah,S F; Green,B N; Wild,B J; Lalloz,M R; Layton,D M

doi:10.3892/ijmm.2.4.451

Oct 1998 Volume 2 Issue 4

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Oct 1998 Volume 2 Issue 4

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Article

Quantification of Ggamma- and Agamma-globins by electrospray ionisation mass spectrometry.

Authors:
- S F Ofori-Acquah
- B N Green
- B J Wild
- M R Lalloz
- D M Layton
View Affiliations / Copyright

Affiliations: Department of Haematological Medicine, King's College School of Medicine and Dentistry, Bessemer Road, London, UK.
Pages: 451-454
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Published online on: October 1, 1998

https://doi.org/10.3892/ijmm.2.4.451
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Abstract

Elucidation of the molecular basis for persistent fetal haemoglobin (Hb F) production in adult life has important implications for the pathophysiology and treatment of human beta haemoglobinopathies. Electrospray ionisation mass spectrometry (ESMS) was applied to analyse the pattern of gamma-globin expression in patients with hereditary persistence of fetal haemoglobin (HPFH) and sickle cell anaemia (SCA). Ggamma and Agamma-globin chains were identified by their measured molecular masses and distinguished by mass difference (14 Da) following deconvolution of ESMS spectra using maximum entropy based software. Prediction of HPFH type by ESMS was confirmed by molecular analysis. Direct determination of Ggamma:Agamma globin chain ratio from whole blood by the novel application of ESMS provides a rapid and sensitive approach to characterisation of gamma-globins and facilitates correlation of gamma-globin level and polymorphism of cis-active elements at the beta-globin locus.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Quantification of Ggamma- and Agamma-globins by electrospray ionisation mass spectrometry.

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Abstract

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