ATP inhibition of proliferation of immortalized human fibroblasts is greater than that of normal human diploid fibroblasts.

Katayama,B; Sakaguchi,M; Li,J W; Pu,H; Inoue,Y; Namba,M

doi:10.3892/ijmm.2.5.603

Nov 1998 Volume 2 Issue 5

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Nov 1998 Volume 2 Issue 5

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Article

ATP inhibition of proliferation of immortalized human fibroblasts is greater than that of normal human diploid fibroblasts.

Authors:
- B Katayama
- M Sakaguchi
- J W Li
- H Pu
- Y Inoue
- M Namba
View Affiliations / Copyright

Affiliations: Department of Cell Biology, Institute of Cellular and Molecular Biology, Okayama University Medical School, Japan.
Pages: 603-609
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Published online on: November 1, 1998

https://doi.org/10.3892/ijmm.2.5.603
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Abstract

It is known that cancers develop by a multi-step process. Normal cells are first immortalized, and then transformed into tumorigenic cells. Normal human cells are very rarely immortalized, but once they are, they are relatively easily transformed into tumorigenic cells. This indicates that the immortalization step plays a critical part in the development of human cancers. Thus, elucidation of the mechanisms of this step would shed light on the process of carcinogenesis in human cells. To understand the causes of immortalization, it is important to determine the differences in cellular phenotype between immortalized and normal human cells. In this study, we found that immortalized human fibroblasts were more sensitive to the growth inhibitory effects of ATP than normal human fibroblasts. ADP was as effective as ATP, but AMP, adenosine, and phosphoric acid were not. These results indicate that a high-energy bound of ATP and ADP may contribute to the growth inhibition of the cells. When the immortalized cells were pulse-labeled with [32P]-ATP, 30-, 31-, 33- and 40-kDa membrane fraction proteins were more prominently labeled in the immortalized cells than in the normal cells. At present, the characteristics of these proteins are being investigated.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

ATP inhibition of proliferation of immortalized human fibroblasts is greater than that of normal human diploid fibroblasts.

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