ATP inhibition of proliferation of immortalized human fibroblasts is greater than that of normal human diploid fibroblasts.

  • Authors:
    • B Katayama
    • M Sakaguchi
    • J W Li
    • H Pu
    • Y Inoue
    • M Namba
  • View Affiliations

  • Published online on: November 1, 1998     https://doi.org/10.3892/ijmm.2.5.603
  • Pages: 603-609
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

It is known that cancers develop by a multi-step process. Normal cells are first immortalized, and then transformed into tumorigenic cells. Normal human cells are very rarely immortalized, but once they are, they are relatively easily transformed into tumorigenic cells. This indicates that the immortalization step plays a critical part in the development of human cancers. Thus, elucidation of the mechanisms of this step would shed light on the process of carcinogenesis in human cells. To understand the causes of immortalization, it is important to determine the differences in cellular phenotype between immortalized and normal human cells. In this study, we found that immortalized human fibroblasts were more sensitive to the growth inhibitory effects of ATP than normal human fibroblasts. ADP was as effective as ATP, but AMP, adenosine, and phosphoric acid were not. These results indicate that a high-energy bound of ATP and ADP may contribute to the growth inhibition of the cells. When the immortalized cells were pulse-labeled with [32P]-ATP, 30-, 31-, 33- and 40-kDa membrane fraction proteins were more prominently labeled in the immortalized cells than in the normal cells. At present, the characteristics of these proteins are being investigated.

Related Articles

Journal Cover

Nov 1998
Volume 2 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Katayama B, Sakaguchi M, Li J, Pu H, Inoue Y and Namba M: ATP inhibition of proliferation of immortalized human fibroblasts is greater than that of normal human diploid fibroblasts.. Int J Mol Med 2: 603-609, 1998
APA
Katayama, B., Sakaguchi, M., Li, J., Pu, H., Inoue, Y., & Namba, M. (1998). ATP inhibition of proliferation of immortalized human fibroblasts is greater than that of normal human diploid fibroblasts.. International Journal of Molecular Medicine, 2, 603-609. https://doi.org/10.3892/ijmm.2.5.603
MLA
Katayama, B., Sakaguchi, M., Li, J., Pu, H., Inoue, Y., Namba, M."ATP inhibition of proliferation of immortalized human fibroblasts is greater than that of normal human diploid fibroblasts.". International Journal of Molecular Medicine 2.5 (1998): 603-609.
Chicago
Katayama, B., Sakaguchi, M., Li, J., Pu, H., Inoue, Y., Namba, M."ATP inhibition of proliferation of immortalized human fibroblasts is greater than that of normal human diploid fibroblasts.". International Journal of Molecular Medicine 2, no. 5 (1998): 603-609. https://doi.org/10.3892/ijmm.2.5.603