Procaspase-3 activation by a metalloprotease secreted from Vibrio vulnificus

  • Authors:
    • Hyo Young Kim
    • Alan K. Chang
    • Jung Eun Park
    • Il-Seon Park
    • Seong Myeong Yoon
    • Jung Sup Lee
  • View Affiliations

  • Published online on: October 1, 2007     https://doi.org/10.3892/ijmm.20.4.591
  • Pages: 591-595
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Abstract

Vibrio vulnificus is a marine bacterium and a human pathogen capable of causing wound infection and septicemia. We previously showed that the metalloprotease vEP secreted by V. vulnificus activates prothrombin in vitro. To further investigate the ability of vEP to activate other zymogens, we used a mutant form of procaspase-3 which lacks the native cleavage sites as a zymogen. The mutant zymogen was activated by vEP to yield a mature enzyme with a maximum increase in caspase-3 activity of approximately 14-fold in a time-dependent manner. However, the increase started to decline with prolonged incubation and with higher protease concentration as a result of further cleavage of the mature enzyme. Western blot analysis revealed a band of ≈17 kDa for the cleavage product, which corresponded with the change in caspase-3 activity. The activated procaspase-3 by vEP was also able to cleave poly(ADP-ribose) polymerase in a cell-free system, and was inhibited by Ac-DEVD-CHO, a potent caspase-3 inhibitor. The results presented are the first to demonstrate the in vitro activation of one of the crucial enzymes involved in cell death by a bacterial extracellular metalloprotease.

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October 2007
Volume 20 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kim HY, Chang AK, Park JE, Park I, Yoon SM and Lee JS: Procaspase-3 activation by a metalloprotease secreted from Vibrio vulnificus. Int J Mol Med 20: 591-595, 2007
APA
Kim, H.Y., Chang, A.K., Park, J.E., Park, I., Yoon, S.M., & Lee, J.S. (2007). Procaspase-3 activation by a metalloprotease secreted from Vibrio vulnificus. International Journal of Molecular Medicine, 20, 591-595. https://doi.org/10.3892/ijmm.20.4.591
MLA
Kim, H. Y., Chang, A. K., Park, J. E., Park, I., Yoon, S. M., Lee, J. S."Procaspase-3 activation by a metalloprotease secreted from Vibrio vulnificus". International Journal of Molecular Medicine 20.4 (2007): 591-595.
Chicago
Kim, H. Y., Chang, A. K., Park, J. E., Park, I., Yoon, S. M., Lee, J. S."Procaspase-3 activation by a metalloprotease secreted from Vibrio vulnificus". International Journal of Molecular Medicine 20, no. 4 (2007): 591-595. https://doi.org/10.3892/ijmm.20.4.591