Interleukin-32α expression in human colonic subepithelial myofibroblasts

  • Authors:
    • Yuhki Yagi
    • Akira Andoh
    • Hirotsugu Imaeda
    • Tomoki Aomatsu
    • Rie Ohsaki
    • Osamu Inatomi
    • Shigeki Bamba
    • Tomoyuki Tsujikawa
    • Tomoharu Shimizu
    • Yoshihide Fujiyama
  • View Affiliations

  • Published online on: December 6, 2010     https://doi.org/10.3892/ijmm.2010.575
  • Pages: 263-268
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Abstract

Interleukin (IL)-32 is a recently described proinflammatory cytokine, characterized by induction of nuclear factor (NF)-κB activation. We studied IL-32α expression in human colonic subepithelial myofibroblasts (SEMFs). Colonic SEMFs were isolated from normal human colon tissue. IL-32α protein expression was evaluated by Western blot analyses, and IL-32α mRNA expression was analyzed by real-time PCR. IL-32α mRNA was weakly expressed without a stimulus, and its expression was markedly enhanced by IL-1ß and TNF-α. IL-1ß and TNF-α enhanced intracellular accumulation of IL-32α protein, but IL-32α was not detected in supernatants. Each cytokine dose- and time-dependently induced IL-32α mRNA expression. An inhibitor of phosphatidylinositol 3-kinase (LY294002) significantly suppressed IL-1ß- and TNF-α-induced IL-32α mRNA expression, although MAPK inhibitors had no effect. Akt activation in response to these cytokines was confirmed by Western blotting. Blockade of NF-κB activation by an adenovirus expressing a stable mutant form of IκBα markedly suppressed IL-1ß- and TNF-α-induced IL-32α mRNA expression. Human colonic SEMFs expressed IL-32α in response to IL-1ß and TNF-α. IL-32α mRNA expression depends on the phosphatidylinositol 3-kinase and the NF-κB system.

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February 2011
Volume 27 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yagi Y, Andoh A, Imaeda H, Aomatsu T, Ohsaki R, Inatomi O, Bamba S, Tsujikawa T, Shimizu T, Fujiyama Y, Fujiyama Y, et al: Interleukin-32α expression in human colonic subepithelial myofibroblasts. Int J Mol Med 27: 263-268, 2011
APA
Yagi, Y., Andoh, A., Imaeda, H., Aomatsu, T., Ohsaki, R., Inatomi, O. ... Fujiyama, Y. (2011). Interleukin-32α expression in human colonic subepithelial myofibroblasts. International Journal of Molecular Medicine, 27, 263-268. https://doi.org/10.3892/ijmm.2010.575
MLA
Yagi, Y., Andoh, A., Imaeda, H., Aomatsu, T., Ohsaki, R., Inatomi, O., Bamba, S., Tsujikawa, T., Shimizu, T., Fujiyama, Y."Interleukin-32α expression in human colonic subepithelial myofibroblasts". International Journal of Molecular Medicine 27.2 (2011): 263-268.
Chicago
Yagi, Y., Andoh, A., Imaeda, H., Aomatsu, T., Ohsaki, R., Inatomi, O., Bamba, S., Tsujikawa, T., Shimizu, T., Fujiyama, Y."Interleukin-32α expression in human colonic subepithelial myofibroblasts". International Journal of Molecular Medicine 27, no. 2 (2011): 263-268. https://doi.org/10.3892/ijmm.2010.575