Human mesangial cells resist glycoxidative stress through an antioxidant response

Nitti,Mariapaola; Furfaro,Anna   Lisa; Patriarca,Stefania; Balbis,Emanuela; Domenicotti,Cinzia; Cottalasso,Damiano; Pronzato,Maria   Adelaide; Marinari,Umberto   Maria; Traverso,Nicola

doi:10.3892/ijmm.2010.576

February 2011 Volume 27 Issue 2

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February 2011 Volume 27 Issue 2

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Article

Human mesangial cells resist glycoxidative stress through an antioxidant response

Authors:
- Mariapaola Nitti
- Anna Lisa Furfaro
- Stefania Patriarca
- Emanuela Balbis
- Cinzia Domenicotti
- Damiano Cottalasso
- Maria Adelaide Pronzato
- Umberto Maria Marinari
- Nicola Traverso
View Affiliations / Copyright

Affiliations: Department of Experimental Medicine, Section of General Pathology, University of Genoa, 16132 Genoa, Italy, DIMES, Section of General Pathology, Via LB Alberti 2, 16132 Genoa, Italy
Pages: 213-219
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Published online on: December 6, 2010

https://doi.org/10.3892/ijmm.2010.576
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Abstract

The generation of advanced glycation end-products (AGE), the interaction with their receptors, the generation of reactive oxygen species, and the modulation of intracellular redox equilibrium are believed to be the main factors causing alterations of mesangial cell physiology leading to diabetic nephropathy. Normal human primary mesangial cells were exposed to glycoxidative stress by culture in high glucose (HG) or treatment with AGE for up to 6 days. In both cases only a moderate generation of reactive oxygen species and production of HNE-protein adducts were induced while protein nitrotyrosination was not affected. Moreover, HG and AGE caused a significant antioxidant response, confirmed by the induction of heme oxygenase 1 and the consumption of vitamin E. Glutathione was decreased only by HG. Mesangial cell proliferation and viability were slightly affected by HG and AGE. Furthermore, both treatments failed to influence TGF-ß1 and MCP-1 secretion and to modulate RAGE and collagen IV expression. We believe that normal human mesangial cells can resist glycoxidative stress by the observed antioxidant response. These results support the concept that mesangial cells are only partly responsible for the onset and progression of diabetic nephropathy and that the role of other cell types, such as podocytes and endothelial cells, should be taken into consideration.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Human mesangial cells resist glycoxidative stress through an antioxidant response

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Abstract

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