An ethanol extract of Iris nertschinskia induces p53-dependent apoptosis in the MCF7 human breast cancer cell line

  • Authors:
    • Jae-Sik Shin
    • Seung-Woo Hong
    • Jin-Gyun Lee
    • Young-Min Lee
    • Dae-Won Kim
    • Jee-Eun Kim
    • Da-Jung Jung
    • Sung-Kwan An
    • Nam-Joo Hong
    • Daejin Kim
    • Dong-Hoon Jin
    • Sok-Young Lee
  • View Affiliations

  • Published online on: January 14, 2011     https://doi.org/10.3892/ijmm.2011.599
  • Pages: 401-405
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Abstract

Iris nertschinskia, an ornamental plant, is utilized in traditional East Asian medicine for the treatment of skin diseases. However, the biological activity underlying its therapeutic effects remains to be established. In this study, we investigated the anti-tumor effect of the plant extract on MCF7 human breast cancer cells. An ethanol extract of Iris nertschinskia triggered cell death in a dose-dependent manner. Moreover, treatment with the extract promoted p53 phosphorylation in MCF7 cells. Increased phosphorylation of p53, in turn, led to induction of Bax protein, a key regulator of p53-dependent apoptotic cell death, as well as of caspase-7 cleavage in MCF7 cells. Consistently, cells treated with p53-specific siRNA or the caspase inhibitor, Z-VAD, resisted apoptotic cell death induced by the Iris nertschinskia extract. Our results suggest that p53 sensitizes tumor cells to the ethanol extract of Iris nertschinskia by Bax protein induction and caspase-dependent apoptosis.

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March 2011
Volume 27 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Shin J, Hong S, Lee J, Lee Y, Kim D, Kim J, Jung D, An S, Hong N, Kim D, Kim D, et al: An ethanol extract of Iris nertschinskia induces p53-dependent apoptosis in the MCF7 human breast cancer cell line. Int J Mol Med 27: 401-405, 2011.
APA
Shin, J., Hong, S., Lee, J., Lee, Y., Kim, D., Kim, J. ... Lee, S. (2011). An ethanol extract of Iris nertschinskia induces p53-dependent apoptosis in the MCF7 human breast cancer cell line. International Journal of Molecular Medicine, 27, 401-405. https://doi.org/10.3892/ijmm.2011.599
MLA
Shin, J., Hong, S., Lee, J., Lee, Y., Kim, D., Kim, J., Jung, D., An, S., Hong, N., Kim, D., Jin, D., Lee, S."An ethanol extract of Iris nertschinskia induces p53-dependent apoptosis in the MCF7 human breast cancer cell line". International Journal of Molecular Medicine 27.3 (2011): 401-405.
Chicago
Shin, J., Hong, S., Lee, J., Lee, Y., Kim, D., Kim, J., Jung, D., An, S., Hong, N., Kim, D., Jin, D., Lee, S."An ethanol extract of Iris nertschinskia induces p53-dependent apoptosis in the MCF7 human breast cancer cell line". International Journal of Molecular Medicine 27, no. 3 (2011): 401-405. https://doi.org/10.3892/ijmm.2011.599