cAMP regulates ADP-induced HSP27 phosphorylation in human platelets

  • Authors:
    • Yukiko Enomoto
    • Seiji Adachi
    • Tomoaki Doi
    • Hideo Natsume
    • Kenji Kato
    • Rie Matsushima-Nishiwaki
    • Shigeru Akamatsu
    • Haruhiko Tokuda
    • Shinichi Yoshimura
    • Takanobu Otsuka
    • Shinji Ogura
    • Osamu Kozawa
    • Toru Iwama
  • View Affiliations

  • Published online on: March 3, 2011     https://doi.org/10.3892/ijmm.2011.637
  • Pages: 695-700
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Abstract

Elevation of cAMP in platelets is recognized to play a suppressive role in platelet functions. We have previously shown that adenosine diphosphate (ADP)-induced phosphorylation of heat shock protein 27 (HSP27) via p38 mitogen-activated protein (MAP) kinase is correlated with platelet-derived growth factor (PDGF)-AB secretion and soluble CD40 ligand (sCD40L) release. In the present study, we investigated the relationship between cAMP and HSP27 phosphorylation in platelet function. 8-Bromoadenosine-3',5'-cyclic monophosphate (8-bromo-cAMP), a plasma membrane-permeable cAMP analogue, or cilostazol, an inhibitor of cAMP phosphodiesterase, markedly attenuated the ADP-induced phosphorylation levels of p38 MAP kinase. In addition, the ADP-induced HSP27 phosphorylation was suppressed by 8-bromo-cAMP or cilostazol. 8-Bromo-cAMP, forskolin and cilostazol remarkably reduced the ADP-stimulated PDGF-AB secretion and sCD40L release. These results strongly suggest that cAMP regulates ADP-stimulated platelet activation due to inhibition of HSP27 phosphorylation via p38 MAP kinase.

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May 2011
Volume 27 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Enomoto Y, Adachi S, Doi T, Natsume H, Kato K, Matsushima-Nishiwaki R, Akamatsu S, Tokuda H, Yoshimura S, Otsuka T, Otsuka T, et al: cAMP regulates ADP-induced HSP27 phosphorylation in human platelets. Int J Mol Med 27: 695-700, 2011
APA
Enomoto, Y., Adachi, S., Doi, T., Natsume, H., Kato, K., Matsushima-Nishiwaki, R. ... Iwama, T. (2011). cAMP regulates ADP-induced HSP27 phosphorylation in human platelets. International Journal of Molecular Medicine, 27, 695-700. https://doi.org/10.3892/ijmm.2011.637
MLA
Enomoto, Y., Adachi, S., Doi, T., Natsume, H., Kato, K., Matsushima-Nishiwaki, R., Akamatsu, S., Tokuda, H., Yoshimura, S., Otsuka, T., Ogura, S., Kozawa, O., Iwama, T."cAMP regulates ADP-induced HSP27 phosphorylation in human platelets". International Journal of Molecular Medicine 27.5 (2011): 695-700.
Chicago
Enomoto, Y., Adachi, S., Doi, T., Natsume, H., Kato, K., Matsushima-Nishiwaki, R., Akamatsu, S., Tokuda, H., Yoshimura, S., Otsuka, T., Ogura, S., Kozawa, O., Iwama, T."cAMP regulates ADP-induced HSP27 phosphorylation in human platelets". International Journal of Molecular Medicine 27, no. 5 (2011): 695-700. https://doi.org/10.3892/ijmm.2011.637