Differential expression of microRNAs in cardiac myocytes compared to undifferentiated P19 cells

  • Authors:
    • De-Liang Hu
    • Yao-Qiu Liu
    • Fu-Kun Chen
    • Yan-Hui Sheng
    • Rong Yang
    • Xiang-Qing Kong
    • Ke-Jiang Cao
    • Jin-Song Zhang
    • Ling-Mei Qian
  • View Affiliations

  • Published online on: March 31, 2011     https://doi.org/10.3892/ijmm.2011.664
  • Pages: 59-64
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Abstract

microRNA (miRNA) expression is tightly controlled in a tissue-specific and developmental stage-specific manner; some are highly and specifically expressed in cardiovascular tissues. miRNA expression profiling, using miRNA microarrays facilitates studying the biological function of miRNAs. We investigated changes in miRNA expression profiles during differentiation of P19 cells into cardiac myocytes in order to elucidate the mechanisms of heart development. The morphology of P19 cells during differentiation was observed using an inverted microscope. Western blot analysis was performed to detect cardiac troponin I (cTnI) expression. Total RNA was extracted from P19 cells for microarray and real-time quantitative reverse transcription-polymerase chain reaction (real-time qRT-PCR) analyses to determine the miRNA expression profile. The miRNA microarray revealed differential expression of 49 miRNAs, of which 26 were down-regulated and 23 were up-regulated in differentiated cardiac myocytes, compared to normal P19 cells. This was confirmed by real-time qRT-PCR. We also utilized target prediction analysis to identify gene targets. Some miRNAs may have important roles in cardiac development and congenital heart defects (CHDs). Further analysis of miRNA function to confirm their target genes during cardiac development will determine the potential for novel miRNA-based therapeutic strategies.

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July 2011
Volume 28 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hu D, Liu Y, Chen F, Sheng Y, Yang R, Kong X, Cao K, Zhang J and Qian L: Differential expression of microRNAs in cardiac myocytes compared to undifferentiated P19 cells. Int J Mol Med 28: 59-64, 2011
APA
Hu, D., Liu, Y., Chen, F., Sheng, Y., Yang, R., Kong, X. ... Qian, L. (2011). Differential expression of microRNAs in cardiac myocytes compared to undifferentiated P19 cells. International Journal of Molecular Medicine, 28, 59-64. https://doi.org/10.3892/ijmm.2011.664
MLA
Hu, D., Liu, Y., Chen, F., Sheng, Y., Yang, R., Kong, X., Cao, K., Zhang, J., Qian, L."Differential expression of microRNAs in cardiac myocytes compared to undifferentiated P19 cells". International Journal of Molecular Medicine 28.1 (2011): 59-64.
Chicago
Hu, D., Liu, Y., Chen, F., Sheng, Y., Yang, R., Kong, X., Cao, K., Zhang, J., Qian, L."Differential expression of microRNAs in cardiac myocytes compared to undifferentiated P19 cells". International Journal of Molecular Medicine 28, no. 1 (2011): 59-64. https://doi.org/10.3892/ijmm.2011.664