Modulatory activities of Agelanthus dodoneifolius (Loranthaceae) extracts on stimulated equine neutrophils and myeloperoxidase activity

  • Authors:
    • Raïnatou Boly
    • Stéphanie Dessy
    • Stephan Kohnen
    • Félix Kini
    • Marius Lompo
    • Ange Mouithys-Mickalad
    • Innocent Pierre Guissou
    • Jacques Dubois
    • Ginette Deby-Dupont
    • Didier Serteyn
    • Thierry Franck
  • View Affiliations

  • Published online on: May 9, 2011     https://doi.org/10.3892/ijmm.2011.695
  • Pages: 261-270
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Abstract

Agelanthus dodoneifolius DC Danser (Loranthaceae) is used for the treatment of various diseases including asthma. The aqueous and hydroalcoholic extracts have been reported to have anti-inflammatory, spasmolytic and bronchorelaxant activities. The present study investigates the effects of the aqueous decoction and the diethyl ether, ethyl acetate and butanolic fractions of Agelanthus dodoneifolius DC Danser (Loranthaceae) on reactive oxygen species (ROS) production and myeloperoxidase (MPO) release by phorbol 12-myristate 13-acetate (PMA)-stimulated equine neutrophils and on purified equine MPO activity. ROS production and MPO release by the PMA-stimulated neutrophils were measured by the lucigenin-enhanced chemiluminescence and ELISA assays, respectively. Specific immunological extraction followed by enzymatic detection (SIEFED) was used to specifically measure the equine MPO activity. Identification and quantification of the individual and total phenolic and flavonoid compounds were performed using UPLC-MS/MS equipment and colorimetric methods involving Folin-Ciocalteu and AlCl3, respectively. All the tested extracts displayed dose-dependent inhibitory effects on the oxidant activities of neutrophils; a stronger effect was observed with the organic fractions than the aqueous decoction. These findings could be correlated with a high content of phenolic and flavonoid compounds. The results confirm the previously shown anti-inflammatory effect of Agelanthus dodoneifolius and its potential use for the treatment of neutrophil-dependent inflammatory diseases.

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August 2011
Volume 28 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Boly R, Dessy S, Kohnen S, Kini F, Lompo M, Mouithys-Mickalad A, Guissou IP, Dubois J, Deby-Dupont G, Serteyn D, Serteyn D, et al: Modulatory activities of Agelanthus dodoneifolius (Loranthaceae) extracts on stimulated equine neutrophils and myeloperoxidase activity. Int J Mol Med 28: 261-270, 2011
APA
Boly, R., Dessy, S., Kohnen, S., Kini, F., Lompo, M., Mouithys-Mickalad, A. ... Franck, T. (2011). Modulatory activities of Agelanthus dodoneifolius (Loranthaceae) extracts on stimulated equine neutrophils and myeloperoxidase activity. International Journal of Molecular Medicine, 28, 261-270. https://doi.org/10.3892/ijmm.2011.695
MLA
Boly, R., Dessy, S., Kohnen, S., Kini, F., Lompo, M., Mouithys-Mickalad, A., Guissou, I. P., Dubois, J., Deby-Dupont, G., Serteyn, D., Franck, T."Modulatory activities of Agelanthus dodoneifolius (Loranthaceae) extracts on stimulated equine neutrophils and myeloperoxidase activity". International Journal of Molecular Medicine 28.2 (2011): 261-270.
Chicago
Boly, R., Dessy, S., Kohnen, S., Kini, F., Lompo, M., Mouithys-Mickalad, A., Guissou, I. P., Dubois, J., Deby-Dupont, G., Serteyn, D., Franck, T."Modulatory activities of Agelanthus dodoneifolius (Loranthaceae) extracts on stimulated equine neutrophils and myeloperoxidase activity". International Journal of Molecular Medicine 28, no. 2 (2011): 261-270. https://doi.org/10.3892/ijmm.2011.695