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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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October 2011 Volume 28 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Capture of dengue virus type 3 using anionic polymer-coated magnetic beads

  • Authors:
    • Akikazu Sakudo
    • Promsin Masrinoul
    • Yasuharu Tanaka
    • Kazuyoshi Ikuta

  • View Affiliations / Copyright

    Affiliations: Laboratory of Biometabolic Chemistry, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan
  • Pages: 625-628
    |
    Published online on: June 27, 2011
       https://doi.org/10.3892/ijmm.2011.733
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Abstract

Dengue virus (DENV) is a mosquito-borne virus and can be transmitted to humans by mosquito vectors. Although surveillance of dengue virus-infected mosquitoes is the most effective way of controlling DENV infections, detection of DENVs in mosquitoes is limited by the low sensitivity of available detection methods. We here report a method for capturing DENV type 3 (DENV-3) from mosquito cells using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate). The beads were incubated with cell culture medium of DENV-3-infected mosquito cells, then separated from the supernatant by applying a magnetic field and washed. Adsorption of DENV-3 on the beads was confirmed by reverse transcription-polymerase chain reaction, which detected the presence of DENV-3 genomic RNA on the beads, and Western blotting, which determined the major DENV-3 envelope protein on the beads. Therefore, this capture method may enable an improvement in DENV-3 detection.

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Copy and paste a formatted citation
Spandidos Publications style
Sakudo A, Masrinoul P, Tanaka Y and Ikuta K: Capture of dengue virus type 3 using anionic polymer-coated magnetic beads. Int J Mol Med 28: 625-628, 2011.
APA
Sakudo, A., Masrinoul, P., Tanaka, Y., & Ikuta, K. (2011). Capture of dengue virus type 3 using anionic polymer-coated magnetic beads. International Journal of Molecular Medicine, 28, 625-628. https://doi.org/10.3892/ijmm.2011.733
MLA
Sakudo, A., Masrinoul, P., Tanaka, Y., Ikuta, K."Capture of dengue virus type 3 using anionic polymer-coated magnetic beads". International Journal of Molecular Medicine 28.4 (2011): 625-628.
Chicago
Sakudo, A., Masrinoul, P., Tanaka, Y., Ikuta, K."Capture of dengue virus type 3 using anionic polymer-coated magnetic beads". International Journal of Molecular Medicine 28, no. 4 (2011): 625-628. https://doi.org/10.3892/ijmm.2011.733
Copy and paste a formatted citation
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Spandidos Publications style
Sakudo A, Masrinoul P, Tanaka Y and Ikuta K: Capture of dengue virus type 3 using anionic polymer-coated magnetic beads. Int J Mol Med 28: 625-628, 2011.
APA
Sakudo, A., Masrinoul, P., Tanaka, Y., & Ikuta, K. (2011). Capture of dengue virus type 3 using anionic polymer-coated magnetic beads. International Journal of Molecular Medicine, 28, 625-628. https://doi.org/10.3892/ijmm.2011.733
MLA
Sakudo, A., Masrinoul, P., Tanaka, Y., Ikuta, K."Capture of dengue virus type 3 using anionic polymer-coated magnetic beads". International Journal of Molecular Medicine 28.4 (2011): 625-628.
Chicago
Sakudo, A., Masrinoul, P., Tanaka, Y., Ikuta, K."Capture of dengue virus type 3 using anionic polymer-coated magnetic beads". International Journal of Molecular Medicine 28, no. 4 (2011): 625-628. https://doi.org/10.3892/ijmm.2011.733
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