Capture of dengue virus type 3 using anionic polymer-coated magnetic beads

  • Authors:
    • Akikazu Sakudo
    • Promsin Masrinoul
    • Yasuharu Tanaka
    • Kazuyoshi Ikuta
  • View Affiliations

  • Published online on: June 27, 2011     https://doi.org/10.3892/ijmm.2011.733
  • Pages: 625-628
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Abstract

Dengue virus (DENV) is a mosquito-borne virus and can be transmitted to humans by mosquito vectors. Although surveillance of dengue virus-infected mosquitoes is the most effective way of controlling DENV infections, detection of DENVs in mosquitoes is limited by the low sensitivity of available detection methods. We here report a method for capturing DENV type 3 (DENV-3) from mosquito cells using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate). The beads were incubated with cell culture medium of DENV-3-infected mosquito cells, then separated from the supernatant by applying a magnetic field and washed. Adsorption of DENV-3 on the beads was confirmed by reverse transcription-polymerase chain reaction, which detected the presence of DENV-3 genomic RNA on the beads, and Western blotting, which determined the major DENV-3 envelope protein on the beads. Therefore, this capture method may enable an improvement in DENV-3 detection.

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October 2011
Volume 28 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sakudo A, Masrinoul P, Tanaka Y and Ikuta K: Capture of dengue virus type 3 using anionic polymer-coated magnetic beads. Int J Mol Med 28: 625-628, 2011.
APA
Sakudo, A., Masrinoul, P., Tanaka, Y., & Ikuta, K. (2011). Capture of dengue virus type 3 using anionic polymer-coated magnetic beads. International Journal of Molecular Medicine, 28, 625-628. https://doi.org/10.3892/ijmm.2011.733
MLA
Sakudo, A., Masrinoul, P., Tanaka, Y., Ikuta, K."Capture of dengue virus type 3 using anionic polymer-coated magnetic beads". International Journal of Molecular Medicine 28.4 (2011): 625-628.
Chicago
Sakudo, A., Masrinoul, P., Tanaka, Y., Ikuta, K."Capture of dengue virus type 3 using anionic polymer-coated magnetic beads". International Journal of Molecular Medicine 28, no. 4 (2011): 625-628. https://doi.org/10.3892/ijmm.2011.733