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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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November 2011 Volume 28 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Cobalt protoporphyrin induces differentiation of monocytic THP-1 cells through regulation of cytoplasmic Ref-1-related NADPH oxidase activity

  • Authors:
    • Ju Dong Song
    • Sang Kwon Lee
    • Si Eun Park
    • Kang Mi Kim
    • Koanhoi Kim
    • Yeong Min Park
    • Young Chul Park

  • View Affiliations / Copyright

    Affiliations: Department of Microbiology and Immunology, Pusan National University School of Medicine, Yangsan, Gyeongnam, Republic of Korea, Department of Micro­biology and Immunology, Pusan National University School of Medicine, Yangsan, Gyeongnam 626-870, Republic of Korea
  • Pages: 841-845
    |
    Published online on: June 27, 2011
       https://doi.org/10.3892/ijmm.2011.736
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Abstract

Cobalt protoporphyrin (CoPP) is a potent and effective metalloporphyrin inducer of heme oxygenase-1 (HO-1) activity in many tissues. Here, we report that CoPP induces differentiation of monocytic THP-1 cells into macrophage-like cells. CoPP induced a marked growth inhibition with a slight reduction in viability, and increased adhesion and spreading of THP-1 cells. However, other protoporphyrins did not. CoPP also resulted in expression of CD11b, MMP9, MSR1, CD14 and ICAM-1, which are differentiation markers for macrophages. Interestingly, we observed a decrease of cytoplasmic redox factor-1 (Ref-1) levels in the process of CoPP-induced differentiation of THP-1 cells. In addition, knockdown of Ref-1 by siRNA enhanced cell adhesion induced by CoPP. Furthermore, an inhibitor of NADPH oxidase, diphenyleneiodonium (DPI), completely abolished CoPP-induced adhesion of Ref-1-deficient cells using an siRNA. A cytosolic factor for NADPH oxidase activity, p47phox, was significantly increased in THP-1 cells by CoPP treatment. Κnockdown of Ref-1 increased CoPP-induced p47phox expression in THP-1 cells. Taken together, these results suggest that CoPP induces differentiation of monocytic THP-1 cells, and that the CoPP-induced differentiation is associated with cytoplasmic Ref-1-related NADPH oxidase activity.

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Copy and paste a formatted citation
Spandidos Publications style
Song JD, Lee SK, Park SE, Kim KM, Kim K, Park YM and Park YC: Cobalt protoporphyrin induces differentiation of monocytic THP-1 cells through regulation of cytoplasmic Ref-1-related NADPH oxidase activity. Int J Mol Med 28: 841-845, 2011.
APA
Song, J.D., Lee, S.K., Park, S.E., Kim, K.M., Kim, K., Park, Y.M., & Park, Y.C. (2011). Cobalt protoporphyrin induces differentiation of monocytic THP-1 cells through regulation of cytoplasmic Ref-1-related NADPH oxidase activity. International Journal of Molecular Medicine, 28, 841-845. https://doi.org/10.3892/ijmm.2011.736
MLA
Song, J. D., Lee, S. K., Park, S. E., Kim, K. M., Kim, K., Park, Y. M., Park, Y. C."Cobalt protoporphyrin induces differentiation of monocytic THP-1 cells through regulation of cytoplasmic Ref-1-related NADPH oxidase activity". International Journal of Molecular Medicine 28.5 (2011): 841-845.
Chicago
Song, J. D., Lee, S. K., Park, S. E., Kim, K. M., Kim, K., Park, Y. M., Park, Y. C."Cobalt protoporphyrin induces differentiation of monocytic THP-1 cells through regulation of cytoplasmic Ref-1-related NADPH oxidase activity". International Journal of Molecular Medicine 28, no. 5 (2011): 841-845. https://doi.org/10.3892/ijmm.2011.736
Copy and paste a formatted citation
x
Spandidos Publications style
Song JD, Lee SK, Park SE, Kim KM, Kim K, Park YM and Park YC: Cobalt protoporphyrin induces differentiation of monocytic THP-1 cells through regulation of cytoplasmic Ref-1-related NADPH oxidase activity. Int J Mol Med 28: 841-845, 2011.
APA
Song, J.D., Lee, S.K., Park, S.E., Kim, K.M., Kim, K., Park, Y.M., & Park, Y.C. (2011). Cobalt protoporphyrin induces differentiation of monocytic THP-1 cells through regulation of cytoplasmic Ref-1-related NADPH oxidase activity. International Journal of Molecular Medicine, 28, 841-845. https://doi.org/10.3892/ijmm.2011.736
MLA
Song, J. D., Lee, S. K., Park, S. E., Kim, K. M., Kim, K., Park, Y. M., Park, Y. C."Cobalt protoporphyrin induces differentiation of monocytic THP-1 cells through regulation of cytoplasmic Ref-1-related NADPH oxidase activity". International Journal of Molecular Medicine 28.5 (2011): 841-845.
Chicago
Song, J. D., Lee, S. K., Park, S. E., Kim, K. M., Kim, K., Park, Y. M., Park, Y. C."Cobalt protoporphyrin induces differentiation of monocytic THP-1 cells through regulation of cytoplasmic Ref-1-related NADPH oxidase activity". International Journal of Molecular Medicine 28, no. 5 (2011): 841-845. https://doi.org/10.3892/ijmm.2011.736
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