Id1 induces tubulogenesis by regulating endothelial cell adhesion and cytoskeletal organization through β1-integrin and Rho-kinase signalling

  • Authors:
    • Juhui Qiu
    • Guixue Wang
    • Qin Peng
    • Jianjun Hu
    • Xiangdong Luo
    • Yiming Zheng
    • Yanqun Teng
    • Chaojun Tang
  • View Affiliations

  • Published online on: July 4, 2011     https://doi.org/10.3892/ijmm.2011.741
  • Pages: 543-548
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Abstract

The inhibitor of differentiation 1 (Id1) protein is required for tubulogenesis, but the molecular signalling pathways remain unclear. Overexpression (Id1-t) or down-regulation (si-Id1) of Id-1 in cell lines, were used to study the function of Id1. The expression of Id1 and β1-integrin was assessed by Western blotting. Up-regulation of Id1 in human umbilical vascular endothelial cells (HUVECs) activated the expression of β1-integrin and promoted cell adhesion and spreading. Conversely, down-regulation of Id1 suppressed β1-integrin expression and inhibited tubulogenesis. By using a β1-integrin antibody to inhibit β1-integrin function, we demonstrated that Id1-induced cell adhesion and tubulogenesis were mediated by β1-integrin. In addition, HUVECs overexpressing Id1 were able to promote capillary tube formation through cytoskeleton reorganization and cell contraction. Finally, the Rho-kinase inhibitor Y27632 inhibited tubulo-genesis induced by Id1. Our findings provide evidence that Id1 regulates tubulogenesis in vitro through β1-integrin and Rho-kinase signalling.

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October 2011
Volume 28 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Qiu J, Wang G, Peng Q, Hu J, Luo X, Zheng Y, Teng Y and Tang C: Id1 induces tubulogenesis by regulating endothelial cell adhesion and cytoskeletal organization through β1-integrin and Rho-kinase signalling. Int J Mol Med 28: 543-548, 2011
APA
Qiu, J., Wang, G., Peng, Q., Hu, J., Luo, X., Zheng, Y. ... Tang, C. (2011). Id1 induces tubulogenesis by regulating endothelial cell adhesion and cytoskeletal organization through β1-integrin and Rho-kinase signalling. International Journal of Molecular Medicine, 28, 543-548. https://doi.org/10.3892/ijmm.2011.741
MLA
Qiu, J., Wang, G., Peng, Q., Hu, J., Luo, X., Zheng, Y., Teng, Y., Tang, C."Id1 induces tubulogenesis by regulating endothelial cell adhesion and cytoskeletal organization through β1-integrin and Rho-kinase signalling". International Journal of Molecular Medicine 28.4 (2011): 543-548.
Chicago
Qiu, J., Wang, G., Peng, Q., Hu, J., Luo, X., Zheng, Y., Teng, Y., Tang, C."Id1 induces tubulogenesis by regulating endothelial cell adhesion and cytoskeletal organization through β1-integrin and Rho-kinase signalling". International Journal of Molecular Medicine 28, no. 4 (2011): 543-548. https://doi.org/10.3892/ijmm.2011.741