Coordinated increase of miRNA-155 and miRNA-196b expression correlates with the detection of the antigenomic strand of hepatitis C virus in peripheral blood mononuclear cells

  • Authors:
    • Martyna Grek
    • Anna Piekarska
    • Jacek Bartkowiak
    • Wojciech Fendler
    • Jan Kuydowicz
    • Piotr Wroblewski
    • Marek Paradowski
    • Malgorzata Sidorkiewicz
  • View Affiliations

  • Published online on: July 12, 2011     https://doi.org/10.3892/ijmm.2011.748
  • Pages: 875-880
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Abstract

A tight relationship has been revealed between cellular microRNAs (miRNAs) and the course of hepatitis C virus (HCV) replication in human hepatoma cells. Although the detection of the antigenomic HCV RNA strand in peripheral blood mononuclear cells (PBMCs) has provided evidence for viral replication in PBMCs, no reports have shown how miRNAs are affected upon HCV RNA synthesis in PBMCs. The aim of the present study was to assess if and how the expression levels of miRNA-155 and miRNA-196b in PBMCs are related to HCV replication in PBMCs of chronic hepatitis C (CHC) patients. Supporting analyses were performed to evaluate the expression of precursor pri-miR-155 (BIC) and Dicer protein. The genomic and antigenomic HCV RNA strands in PBMCs were detected by strand-specific qRT-PCR. The expression levels of miRNAs, BIC RNA and Dicer protein were assayed on PBMCs by qRT-PCR and Western blotting, respectively. miRNA-155 and miRNA-196b were detected in all studied PBMC samples, but their levels varied according to the presence of the antigenomic HCV RNA strand in PBMCs. Increased expression levels of miRNA-155 and miRNA-196b were associated with the presence of the antigenomic HCV RNA strand in PBMCs. In this group of patients higher frequency of BIC RNA and Dicer protein detection was also found. This study demonstrates that HCV RNA replication in PBMCs of CHC patients is connected with the increased and coordinated expression of miRNA-155 and miRNA-196b.

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November 2011
Volume 28 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Grek M, Piekarska A, Bartkowiak J, Fendler W, Kuydowicz J, Wroblewski P, Paradowski M and Sidorkiewicz M: Coordinated increase of miRNA-155 and miRNA-196b expression correlates with the detection of the antigenomic strand of hepatitis C virus in peripheral blood mononuclear cells. Int J Mol Med 28: 875-880, 2011.
APA
Grek, M., Piekarska, A., Bartkowiak, J., Fendler, W., Kuydowicz, J., Wroblewski, P. ... Sidorkiewicz, M. (2011). Coordinated increase of miRNA-155 and miRNA-196b expression correlates with the detection of the antigenomic strand of hepatitis C virus in peripheral blood mononuclear cells. International Journal of Molecular Medicine, 28, 875-880. https://doi.org/10.3892/ijmm.2011.748
MLA
Grek, M., Piekarska, A., Bartkowiak, J., Fendler, W., Kuydowicz, J., Wroblewski, P., Paradowski, M., Sidorkiewicz, M."Coordinated increase of miRNA-155 and miRNA-196b expression correlates with the detection of the antigenomic strand of hepatitis C virus in peripheral blood mononuclear cells". International Journal of Molecular Medicine 28.5 (2011): 875-880.
Chicago
Grek, M., Piekarska, A., Bartkowiak, J., Fendler, W., Kuydowicz, J., Wroblewski, P., Paradowski, M., Sidorkiewicz, M."Coordinated increase of miRNA-155 and miRNA-196b expression correlates with the detection of the antigenomic strand of hepatitis C virus in peripheral blood mononuclear cells". International Journal of Molecular Medicine 28, no. 5 (2011): 875-880. https://doi.org/10.3892/ijmm.2011.748