ECM-based triple layered scaffolds for vascular tissue engineering

  • Authors:
    • Claudio Grandi
    • Francesca Martorina
    • Silvano Lora
    • Daniele Dalzoppo
    • Pietro Amistà
    • Leonardo Sartore
    • Rosa Di Liddo
    • Maria Teresa Conconi
    • Pier Paolo Parnigotto
  • View Affiliations

  • Published online on: August 11, 2011     https://doi.org/10.3892/ijmm.2011.776
  • Pages: 947-952
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Abstract

The present study focused on the development of three layered small-diameter (<6 mm) extracellular matrix (ECM)-based vessels. These were engineered artificially through the freeze-drying technique. A layer of decellularized bovine aorta (DAM) was deposited on a mandrel and, after lyophilization, it was dipped into a poly-L-lactide acid (PLLA)/polyethylene glycol (PEG) 2000 dichloromethane solution then quickly wrapped with a pre-prepared thin DAM sheet. Mechanical properties of three-layered scaffolds were evaluated by means of uniaxial tensile measurement. Furthermore, human endothelial and smooth muscle cells were seeded on internal and external scaffold surfaces, respectively, and co-cultured for 7 days. Our results demonstrate that i) ECM components provide suitable stimuli for cell adhesion and proliferation, ii) the microporous intermediate PLLA/PEG2000 layer is responsible for the scaffold resistance and iii) the layered deposition technique can be considered a valuable method to obtain layered vascular scaffolds of different sizes and with a good compromise between stiffness and elasticity for optimal cell organization.

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December 2011
Volume 28 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Grandi, C., Martorina, F., Lora, S., Dalzoppo, D., Amistà, P., Sartore, L. ... Parnigotto, P.P. (2011). ECM-based triple layered scaffolds for vascular tissue engineering . International Journal of Molecular Medicine, 28, 947-952. https://doi.org/10.3892/ijmm.2011.776
MLA
Grandi, C., Martorina, F., Lora, S., Dalzoppo, D., Amistà, P., Sartore, L., Di Liddo, R., Conconi, M. T., Parnigotto, P. P."ECM-based triple layered scaffolds for vascular tissue engineering ". International Journal of Molecular Medicine 28.6 (2011): 947-952.
Chicago
Grandi, C., Martorina, F., Lora, S., Dalzoppo, D., Amistà, P., Sartore, L., Di Liddo, R., Conconi, M. T., Parnigotto, P. P."ECM-based triple layered scaffolds for vascular tissue engineering ". International Journal of Molecular Medicine 28, no. 6 (2011): 947-952. https://doi.org/10.3892/ijmm.2011.776