The potential of mouse skin-derived precursors to differentiate into mesenchymal and neural lineages and their application to osteogenic induction in vivo

Kang,Hyun Ki; Min,Seung-Ki; Jung,Sung Youn; Jung,Kyoungsuk; Jang,Da Hyun; Kim,O Bok; Chun,Gae-Sig; Lee,Zang Hee; Min,Byung-Moo

doi:10.3892/ijmm.2011.785

December 2011 Volume 28 Issue 6

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December 2011 Volume 28 Issue 6

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Article

The potential of mouse skin-derived precursors to differentiate into mesenchymal and neural lineages and their application to osteogenic induction in vivo

Authors:
- Hyun Ki Kang
- Seung-Ki Min
- Sung Youn Jung
- Kyoungsuk Jung
- Da Hyun Jang
- O Bok Kim
- Gae-Sig Chun
- Zang Hee Lee
- Byung-Moo Min
View Affiliations / Copyright

Affiliations: Department of Oral Biochemistry and Program in Cancer and Developmental Biology, Seoul National University School of Dentistry, Seoul 110-749, Republic of Korea, Department of Oral and Maxillofacial Surgery, Seoul National University School of Dentistry, Seoul 110-749, Republic of Korea, Department of Cell and Developmental Biology, Seoul National University School of Dentistry, Seoul 110-749, Republic of Korea, Department of Oral Physiology, Dankook University School of Dentistry, Cheonan 330-714, Republic of Korea
Pages: 1001-1011
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Published online on: August 29, 2011

https://doi.org/10.3892/ijmm.2011.785
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Abstract

Although previous studies indicate that skin-derived precursors (SKPs) are multipotent dermal precursors that share similarities with neural crest stem cells (NCSCs), a shared ability for multilineage differentiation toward neural crest lineages between SKPs and NCSCs has not been fully demonstrated. Here, we report the derivation of SKPs from adult mouse skin and their directed multilineage differentiation toward neural crest lineages. Under controlled in vitro conditions, mouse SKPs were propagated and directed toward peripheral nervous system lineages such as peripheral neurons and Schwann cells, and mesenchymal lineages, such as osteogenic, chondrogenic, adipogenic, and smooth muscle cells. To ask if SKPs could generate these same lineages in vivo, a mixture of SKP-derived mesenchymal stem cells and hydroxyapatite/tricalcium phosphate was transplanted into the rat calvarial defects. Over the ensuing 4 weeks, we observed formation of osteogenic structure in the calvarial defect without any evidence of teratomas. These findings demonstrate the multipotency of adult mouse SKPs to differentiate into neural crest lineages. In addition, SKP-derived mesenchymal stem cells represent an accessible, potentially autologous source of precursor cells for tissue-engineered bone repair.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

The potential of mouse skin-derived precursors to differentiate into mesenchymal and neural lineages and their application to osteogenic induction in vivo

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