Zanthoxylum avicennae extracts inhibit cell proliferation through protein phosphatase 2A activation in HA22T human hepatocellular carcinoma cells in vitro and in vivo

Dung,Tran  Duc; Chang,Hsien-Cheh; Binh,Truong  Viet; Lee,Maw-Rong; Tsai,Chang-Hai; Tsai,Fuu-Jen; Kuo,Wei-Wen; Chen,Li-Mien; Huang,Chih-Yang

doi:10.3892/ijmm.2012.938

June 2012 Volume 29 Issue 6

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June 2012 Volume 29 Issue 6

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Article

Zanthoxylum avicennae extracts inhibit cell proliferation through protein phosphatase 2A activation in HA22T human hepatocellular carcinoma cells in vitro and in vivo

Authors:
- Tran Duc Dung
- Hsien-Cheh Chang
- Truong Viet Binh
- Maw-Rong Lee
- Chang-Hai Tsai
- Fuu-Jen Tsai
- Wei-Wen Kuo
- Li-Mien Chen
- Chih-Yang Huang
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Affiliations: School of Chinese Medicine, China Medical University, Taichung, Taiwan, R.O.C., School of Chinese Medicine, Vietnam Academy of Traditional Medicine, Ha Noi, Vietnam, Department of Chemistry, National Chung Hsing University, Taichung, Taiwan, R.O.C., Department of Healthcare Administration, Asia University, Taichung, Taiwan, R.O.C., Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C., Division of Medical Technology, Department of Internal Medicine, Armed-Force, Taichung General Hospital, Taichung, Taiwan, R.O.C., Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, R.O.C.
Pages: 1045-1052
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Published online on: March 13, 2012

https://doi.org/10.3892/ijmm.2012.938
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Abstract

Hepatocellular carcinoma is a common type of cancer that is usually associated with poor prognosis. In this study, we examined the in vitro and in vivo mechanisms of the traditional Vietnamese herb Zanthoxylum avicennae on the inhibition of HA22T human hepatocellular carcinoma cell proliferation. HA22T cells were treated with different concentrations of Zanthoxylum avicennae extracts (YBBEs) and analyzed with the MTT assay, western blot analysis, flow cytometry, siRNA transfection assays and co-immunoprecipitation assay. Additionally, the HA22T-implanted xenograft nude mouse model was applied to confirm the cellular effects. YBBEs showed a strong inhibition of HA22T cell viability in a dose-dependent manner and significantly reduced cell proliferation-related proteins as well as induced cell cycle arrest in the G2/M phase. Protein phosphatase 2A (PP2A) siRNA or okadaic acid totally blocked YBBE-mediated cell proliferation inhibition. In addition, an HA22T-implanted nude mouse model further confirmed that YBBEs inhibit HA22T tumor cell growth and downregulate the survival and cell cycle regulating proteins, as well as activate the PP2A protein. Our findings indicate that the inhibition of HA22T cell proliferation by YBBEs is mediated through PP2A activation.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Zanthoxylum avicennae extracts inhibit cell proliferation through protein phosphatase 2A activation in HA22T human hepatocellular carcinoma cells in vitro and in vivo

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