Emodin inhibits tumor necrosis factor-α-induced migration and inflammatory responses in rat aortic smooth muscle cells

  • Authors:
    • Liang Meng
    • Dongming Yan
    • Weidong Xu
    • Jian Ma
    • Bin Chen
    • Hong  Feng
  • View Affiliations

  • Published online on: March 15, 2012     https://doi.org/10.3892/ijmm.2012.940
  • Pages: 999-1006
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Emodin, a naturally occurring anthraquinone derivative in oriental herbal medicine, has been shown to exert a variety of pharmacological activities. The goal of this study was to determine the effects of emodin on the modulation of cell proliferation, migration, inflammatory responses, and matrix metalloproteinase (MMP)-2 and MMP-9 expression in tumor necrosis factor (TNF)-α-induced rat aortic smooth muscle cells (RASMCs). Cell proliferation and migration were measured using the MTT assay and the transwell chamber assay, respectively. Quantitative real-time PCR and western blot analysis were used to detect MMP expression. Gel shift was used for analysis of nuclear factor (NF)-κB activation. In addition, the expression of several inflammatory genes was also analyzed. Treatment of RASMCs with emodin significantly and dose-dependently attenuated TNF-α-induced proliferation, migration, mRNA and protein expression of MMP-2 and MMP-9, and NF-κB activation. Furthermore, emodin significantly inhibited TNF-α-evoked inflammatory responses, as demonstrated by the reduction in the expression of inflammatory genes. These results suggest that emodin inhibits TNF-α-induced proliferation, migration, MMP-2 and MMP-9 expression as well as inflammatory responses in cultured RASMCs, supporting the notion that emodin may have potential application in clinical atherosclerosis disease.

Related Articles

Journal Cover

June 2012
Volume 29 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Meng L, Yan D, Xu W, Ma J, Chen B and Feng H: Emodin inhibits tumor necrosis factor-α-induced migration and inflammatory responses in rat aortic smooth muscle cells. Int J Mol Med 29: 999-1006, 2012
APA
Meng, L., Yan, D., Xu, W., Ma, J., Chen, B., & Feng, H. (2012). Emodin inhibits tumor necrosis factor-α-induced migration and inflammatory responses in rat aortic smooth muscle cells. International Journal of Molecular Medicine, 29, 999-1006. https://doi.org/10.3892/ijmm.2012.940
MLA
Meng, L., Yan, D., Xu, W., Ma, J., Chen, B., Feng, H."Emodin inhibits tumor necrosis factor-α-induced migration and inflammatory responses in rat aortic smooth muscle cells". International Journal of Molecular Medicine 29.6 (2012): 999-1006.
Chicago
Meng, L., Yan, D., Xu, W., Ma, J., Chen, B., Feng, H."Emodin inhibits tumor necrosis factor-α-induced migration and inflammatory responses in rat aortic smooth muscle cells". International Journal of Molecular Medicine 29, no. 6 (2012): 999-1006. https://doi.org/10.3892/ijmm.2012.940