Activity of antibacterial protein from maggots against Staphylococcus aureus in vitro and in vivo

  • Authors:
    • Zhen Zhang
    • Jiangning Wang
    • Bo Zhang
    • Huanran Liu
    • Wei Song
    • Jiao He
    • Decheng Lv
    • Shouyu Wang
    • Xiaoguang Xu
  • View Affiliations

  • Published online on: March 5, 2013     https://doi.org/10.3892/ijmm.2013.1291
  • Pages: 1159-1165
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Abstract

Maggots (larvae of Lucilia sericata) have shown therapeutic effects on refractory wounds infected with bacteria, yet the bacterial killing mechanisms are unclear. Herein, we report the isolation and purification of an antibacterial protein from maggots (MAMP). MAMP demonstrated inhibitory activity against both standard strains and clinically isolated antibiotic-resistant strains of Staphylococcus aureus in vitro. The topical use of MAMP effectively decreased the viability of S. aureus and promoted wound healing in an S. aureus mouse skin infection model. MAMP exerted its antibacterial activity via a bactericidal mechanism based on observations using scanning electron and transmission electron microscopy. MAMP interacted with the bacterial cell membrane and disrupted the cell surface structure. In addition, MAMP had weak hemolytic activity at a high concentration. Taken together, MAMP exhibits potential use as a topical agent for treating bacterial infections.
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May 2013
Volume 31 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhang Z, Wang J, Zhang B, Liu H, Song W, He J, Lv D, Wang S and Xu X: Activity of antibacterial protein from maggots against Staphylococcus aureus in vitro and in vivo. Int J Mol Med 31: 1159-1165, 2013.
APA
Zhang, Z., Wang, J., Zhang, B., Liu, H., Song, W., He, J. ... Xu, X. (2013). Activity of antibacterial protein from maggots against Staphylococcus aureus in vitro and in vivo. International Journal of Molecular Medicine, 31, 1159-1165. https://doi.org/10.3892/ijmm.2013.1291
MLA
Zhang, Z., Wang, J., Zhang, B., Liu, H., Song, W., He, J., Lv, D., Wang, S., Xu, X."Activity of antibacterial protein from maggots against Staphylococcus aureus in vitro and in vivo". International Journal of Molecular Medicine 31.5 (2013): 1159-1165.
Chicago
Zhang, Z., Wang, J., Zhang, B., Liu, H., Song, W., He, J., Lv, D., Wang, S., Xu, X."Activity of antibacterial protein from maggots against Staphylococcus aureus in vitro and in vivo". International Journal of Molecular Medicine 31, no. 5 (2013): 1159-1165. https://doi.org/10.3892/ijmm.2013.1291