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Article

Changes in glutamate homeostasis cause retinal degeneration in Royal College of Surgeons rats

  • Authors:
    • Kang Liu
    • Yi Wang
    • Zhengqin Yin
    • Chuanhuang Weng
    • Yuxiao Zeng
  • View Affiliations / Copyright

    Affiliations: Southwest Eye Hospital, Southwest Hospital, The Third Military Medical University, Chongqing 400038, P.R. China
  • Pages: 1075-1080
    |
    Published online on: March 12, 2013
       https://doi.org/10.3892/ijmm.2013.1297
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Abstract

The aim of the present study was to investigate glutamate homeostasis in retinal degeneration-induced changes and the potential mechanisms of glutamate-mediated excitotoxicity in a rat model. The expression of vesicular glutamate transporter-1 (VGLUT-1) and protein kinase Cα (PKCα) in wild-type and Royal College of Surgeons (RCS) rat retinas, at postnatal Day 15 (P15), P30, P60 and P90, were detected using quantitative real-time polymerase chain reaction and immunohistochemistry. The levels of glutamine synthetase (GS) and L-glutamate/L-aspartate transporter (GLAST) were evaluated by western blotting. Compared with wild-type rats, outer nuclear layer thickness was significantly thinner and VGLUT-1 expression was upregulated in a time-dependent pattern in RCS rats. The ratio of VGLUT-1 to PKCα in RCS rats peaked at P60 (p<0.01) and subsequently decreased by P90 (p<0.01), while it remained constant in wild-type rats. The expression of GS increased gradually from P30 to P90 in RCS rats (p<0.01), while it remained constant in wild-type rats at various time-points. No significant difference in GLAST expression was found between RCS and wild-type rats at all stages of retinal degeneration. Our results confirm the occurrence of glutamate-mediated excitotoxicity to RCS rat retinas and provide an experimental foundation for safeguarding the remnant visual function in retinal degenerative disorders.
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1 

Olney JW: The toxic effects of glutamate and related compounds in the retina and the brain. Retina. 2:341–359. 1982. View Article : Google Scholar : PubMed/NCBI

2 

Sullivan LS and Daiger SP: Inherited retinal degeneration: exceptional genetic and clinical heterogeneity. Mol Med Today. 2:380–386. 1996. View Article : Google Scholar : PubMed/NCBI

3 

D’Cruz PM, Yasumura D, Weir J, et al: Mutation of the receptor tyrosine kinase gene Mertk in the retinal dystrophic RCS rat. Hum Mol Genet. 9:645–651. 2000.PubMed/NCBI

4 

LaVail MM: Photoreceptor characteristics in congenic strains of RCS rats. Invest Ophthalmol Vis Sci. 20:671–675. 1981.PubMed/NCBI

5 

LaVail MM, Sidman RL and Gerhardt CO: Congenic strains of RCS rats with inherited retinal dystrophy. J Hered. 66:242–244. 1975.PubMed/NCBI

6 

Strettoi E, Porciatti V, Falsini B, Pignatelli V and Rossi C: Morphological and functional abnormalities in the inner retina of the rd/rd mouse. J Neurosci. 22:5492–5504. 2002.PubMed/NCBI

7 

Strettoi E and Pignatelli V: Modifications of retinal neurons in a mouse model of retinitis pigmentosa. Proc Natl Acad Sci USA. 97:11020–11025. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Santos A, Humayun MS, Juan Ed, et al: Preservation of the inner retina in retinitis pigmentosa. A morphometric analysis. Arch Ophthalmol. 115:511–515. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Jones BW and Marc RE: Retinal remodeling during retinal degeneration. Exp Eye Res. 81:123–137. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Marc RE and Jones BW: Retinal remodeling in inherited photoreceptor degenerations. Mol Neurobiol. 28:139–147. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Chen ZS, Yin ZQ, Chen S and Wang SJ: Electrophysiological changes of retinal ganglion cells in Royal College of Surgeons rats during retinal degeneration. Neuroreport. 16:971–975. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Kalloniatis M and Tomisich G: Amino acid neurochemistry of the vertebrate retina. Prog Retin Eye Res. 18:811–866. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Babai N, Morgans CW and Thoreson WB: Calcium-induced calcium release contributes to synaptic release from mouse rod photoreceptors. Neuroscience. 165:1447–1456. 2010. View Article : Google Scholar

14 

Yang XL: Characterization of receptors for glutamate and GABA in retinal neurons. Prog Neurobiol. 73:127–150. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Karl MO, Hayes S, Nelson BR, Tan K, Buckingham B and Reh TA: Stimulation of neural regeneration in the mouse retina. Proc Natl Acad Sci USA. 105:19508–19513. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Shen W, Li S, Chung SH and Gillies MC: Retinal vascular changes after glial disruption in rats. J Neurosci Res. 88:1485–1499. 2010.PubMed/NCBI

17 

Nicoletti F, Bruno V, Copani A, Casabona G and Knopfel T: Metabotropic glutamate receptors: a new target for the therapy of neurodegenerative disorders? Trends Neurosci. 19:267–271. 1996. View Article : Google Scholar : PubMed/NCBI

18 

Phillips MJ, Otteson DC and Sherry DM: Progression of neuronal and synaptic remodeling in the rd10 mouse model of retinitis pigmentosa. J Comp Neurol. 518:2071–2089. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Cuenca N, Pinilla I, Sauve Y and Lund R: Early changes in synaptic connectivity following progressive photoreceptor degeneration in RCS rats. Eur J Neurosci. 22:1057–1072. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Zhang J and Diamond JS: Subunit- and pathway-specific localization of NMDA receptors and scaffolding proteins at ganglion cell synapses in rat retina. J Neurosci. 29:4274–4286. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Jarsky T, Tian M and Singer JH: Nanodomain control of exocytosis is responsible for the signaling capability of a retinal ribbon synapse. J Neurosci. 30:11885–11895. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Vecino E, Hernandez M and Garcia M: Cell death in the developing vertebrate retina. Int J Dev Biol. 48:965–974. 2004. View Article : Google Scholar

23 

Vogel M and Moller K: Cellular decay in the rat retina during normal post-natal development: a preliminary quantitative analysis of the basic endogenous rhythm. Albrecht Von Graefes Arch Klin Exp Ophthalmol. 212:243–260. 1980. View Article : Google Scholar

24 

Jones BW, Watt CB and Marc RE: Retinal remodelling. Clin Exp Optom. 88:282–291. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Marc RE, Jones BW, Anderson JR, et al: Neural reprogramming in retinal degeneration. Invest Ophth Vis Sci. 48:3364–3371. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Gong J, Jellali A, Mutterer J, Sahel JA, Rendon A and Picaud S: Distribution of vesicular glutamate transporters in rat and human retina. Brain Res. 1082:73–85. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Fremeau RT, Kam K, Qureshi T, et al: Vesicular glutamate transporters 1 and 2 target to functionally distinct synaptic release sites. Science. 304:1815–1819. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Delyfer MN, Forster V, Neveux N, Picaud S, Leveillard T and Sahel JA: Evidence for glutamate-mediated excitotoxic mechanisms during photoreceptor degeneration in the rd1 mouse retina. Mol Vis. 11:688–696. 2005.PubMed/NCBI

29 

Okada M, Okuma Y, Osumi Y, Nishihara M, Yokotani K and Ueno H: Neurotransmitter contents in the retina of RCS rat. Graefes Arch Clin Exp Ophthalmol. 238:998–1001. 2000. View Article : Google Scholar : PubMed/NCBI

30 

Garlin AB, Sinor AD, Sinor JD, Jee SH, Grinspan JB and Robinson MB: Pharmacology of sodium-dependent high-affinity L-[3H]glutamate transport in glial cultures. J Neurochem. 64:2572–2580. 1995.

31 

Minami M, Oku H, Okuno T, Fukuhara M and Ikeda T: High infusion pressure in conjunction with vitreous surgery alters the morphology and function of the retina of rabbits. Acta Ophthalmol Scan. 85:633–639. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Sedaghat K, Finkelstein DI and Gundlach AL: Effect of unilateral lesion of the nigrostriatal dopamine pathway on survival and neurochemistry of parafascicular nucleus neurons in the rat - evaluation of time-course and LGR8 expression. Brain Res. 1271:83–94. 2009. View Article : Google Scholar

33 

Friedman LK and Segal M: Early exposure of cultured hippocampal neurons to excitatory amino acids protects from later excitotoxicity. Int J Dev Neurosci. 28:195–205. 2010. View Article : Google Scholar

34 

Ward MM, Jobling AI, Kalloniatis M and Fletcher EL: Glutamate uptake in retinal glial cells during diabetes. Diabetologia. 48:351–360. 2005. View Article : Google Scholar : PubMed/NCBI

35 

Namekata K, Harada C, Kohyama K, Matsumoto Y and Harada T: Interleukin-1 stimulates glutamate uptake in glial cells by accelerating membrane trafficking of Na+/K+-ATPase via actin depolymerization. Mol Cell Biol. 28:3273–3280. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Otori Y, Shimada S, Tanaka K, Ishimoto I, Tano Y and Tohyama M: Marked increase in glutamate-aspartate transporter (GLAST/GluT-1) mRNA following transient retinal ischemia. Mol Brain Res. 27:310–314. 1994. View Article : Google Scholar : PubMed/NCBI

37 

Derouiche A and Rauen T: Coincidence of L-glutamate/L-aspartate transporter (GLAST) and glutamine synthetase (GS) immunoreactions in retinal glia: evidence for coupling of GLAST and GS in transmitter clearance. J Neurosci Res. 42:131–143. 1995. View Article : Google Scholar : PubMed/NCBI

38 

Lehre KP, Davanger S and Danbolt NC: Localization of the glutamate transporter protein GLAST in rat retina. Brain Res. 744:129–137. 1997. View Article : Google Scholar : PubMed/NCBI

39 

Shen F, Chen B, Danias J, et al: Glutamate-induced glutamine synthetase expression in retinal Müller cells after short-term ocular hypertension in the rat. Invest Ophthalmol Vis Sci. 45:3107–3112. 2004.

40 

Germer A, Jahnke C, Mack A, Enzmann V and Reichenbach A: Modification of glutamine synthetase expression by mammalian Müller (glial) cells in retinal organ cultures. Neuroreport. 8:3067–3072. 1997.

41 

Shen X and Xu G: Role of IL-1beta on the glutamine synthetase in retinal Muller cells under high glucose conditions. Curr Eye Res. 34:727–736. 2009. View Article : Google Scholar : PubMed/NCBI

42 

Jablonski MM, Tombran-Tink J, Mrazek DA and Iannaccone A: Pigment epithelium-derived factor supports normal Müller cell development and glutamine synthetase expression after removal of the retinal pigment epithelium. Glia. 35:14–25. 2001.

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Copy and paste a formatted citation
Spandidos Publications style
Liu K, Wang Y, Yin Z, Weng C and Zeng Y: Changes in glutamate homeostasis cause retinal degeneration in Royal College of Surgeons rats. Int J Mol Med 31: 1075-1080, 2013.
APA
Liu, K., Wang, Y., Yin, Z., Weng, C., & Zeng, Y. (2013). Changes in glutamate homeostasis cause retinal degeneration in Royal College of Surgeons rats. International Journal of Molecular Medicine, 31, 1075-1080. https://doi.org/10.3892/ijmm.2013.1297
MLA
Liu, K., Wang, Y., Yin, Z., Weng, C., Zeng, Y."Changes in glutamate homeostasis cause retinal degeneration in Royal College of Surgeons rats". International Journal of Molecular Medicine 31.5 (2013): 1075-1080.
Chicago
Liu, K., Wang, Y., Yin, Z., Weng, C., Zeng, Y."Changes in glutamate homeostasis cause retinal degeneration in Royal College of Surgeons rats". International Journal of Molecular Medicine 31, no. 5 (2013): 1075-1080. https://doi.org/10.3892/ijmm.2013.1297
Copy and paste a formatted citation
x
Spandidos Publications style
Liu K, Wang Y, Yin Z, Weng C and Zeng Y: Changes in glutamate homeostasis cause retinal degeneration in Royal College of Surgeons rats. Int J Mol Med 31: 1075-1080, 2013.
APA
Liu, K., Wang, Y., Yin, Z., Weng, C., & Zeng, Y. (2013). Changes in glutamate homeostasis cause retinal degeneration in Royal College of Surgeons rats. International Journal of Molecular Medicine, 31, 1075-1080. https://doi.org/10.3892/ijmm.2013.1297
MLA
Liu, K., Wang, Y., Yin, Z., Weng, C., Zeng, Y."Changes in glutamate homeostasis cause retinal degeneration in Royal College of Surgeons rats". International Journal of Molecular Medicine 31.5 (2013): 1075-1080.
Chicago
Liu, K., Wang, Y., Yin, Z., Weng, C., Zeng, Y."Changes in glutamate homeostasis cause retinal degeneration in Royal College of Surgeons rats". International Journal of Molecular Medicine 31, no. 5 (2013): 1075-1080. https://doi.org/10.3892/ijmm.2013.1297
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