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International Journal of Molecular Medicine
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Article

Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments

  • Authors:
    • Chuan Cai
    • Gong-Jie Yuan
    • Ye Huang
    • Nan Yang
    • Xin Chen
    • Li Wen
    • Xi Wang
    • Liang Zhang
    • Yin Ding
  • View Affiliations / Copyright

    Affiliations: Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, P.R. China, Affiliated Hospital of Institute of Aviation Medicine, Air Force, Beijing 100089, P.R. China
  • Pages: 1195-1201
    |
    Published online on: March 15, 2013
       https://doi.org/10.3892/ijmm.2013.1305
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Abstract

Recently, it has been reported that the orphan nuclear receptor estrogen-related receptor α (ERRα) is involved in the osteogenic differentiation of mesenchymal stem cells (MSCs). Moreover, ERRα has been identified as a novel therapeutic target for treating osteoporosis and other bone diseases. Human periodontal ligament tissue-derived mesenchymal stem cells (hPDLSCs) have recently been used in stem cell-mediated therapies because of their multipotency, particularly toward osteogenic differentiation. However, it is still unclear whether ERRα can regulate the osteogenic differentiation of hPDLSCs. In the present study, we investigated the role of ERRα in the osteogenic differentiation of hPDLSCs in vitro. We isolated hPDLSCs and confirmed their capacity for multipotent differentiation. Furthermore, we examined ERRα expression in hPDLSCs by RT-PCR and immunocytochemistry. We found that the expression of ERRα mRNA was significantly increased during the late stage of osteogenic differentiation of hPDLSCs. Moreover, transfection of recombinant lentiviral-mediated miRNA targeting ERRα significantly suppressed ALP activity, mineralization capacity, and the mRNA expression of osteogenesis-related genes (ALP, OCN, RUNX2 and OPN) in hPDLSCs. Our results indicate that ERRα may promote the osteogenic differentiation of hPDLSCs in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Cai C, Yuan G, Huang Y, Yang N, Chen X, Wen L, Wang X, Zhang L and Ding Y: Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments. Int J Mol Med 31: 1195-1201, 2013.
APA
Cai, C., Yuan, G., Huang, Y., Yang, N., Chen, X., Wen, L. ... Ding, Y. (2013). Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments. International Journal of Molecular Medicine, 31, 1195-1201. https://doi.org/10.3892/ijmm.2013.1305
MLA
Cai, C., Yuan, G., Huang, Y., Yang, N., Chen, X., Wen, L., Wang, X., Zhang, L., Ding, Y."Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments". International Journal of Molecular Medicine 31.5 (2013): 1195-1201.
Chicago
Cai, C., Yuan, G., Huang, Y., Yang, N., Chen, X., Wen, L., Wang, X., Zhang, L., Ding, Y."Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments". International Journal of Molecular Medicine 31, no. 5 (2013): 1195-1201. https://doi.org/10.3892/ijmm.2013.1305
Copy and paste a formatted citation
x
Spandidos Publications style
Cai C, Yuan G, Huang Y, Yang N, Chen X, Wen L, Wang X, Zhang L and Ding Y: Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments. Int J Mol Med 31: 1195-1201, 2013.
APA
Cai, C., Yuan, G., Huang, Y., Yang, N., Chen, X., Wen, L. ... Ding, Y. (2013). Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments. International Journal of Molecular Medicine, 31, 1195-1201. https://doi.org/10.3892/ijmm.2013.1305
MLA
Cai, C., Yuan, G., Huang, Y., Yang, N., Chen, X., Wen, L., Wang, X., Zhang, L., Ding, Y."Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments". International Journal of Molecular Medicine 31.5 (2013): 1195-1201.
Chicago
Cai, C., Yuan, G., Huang, Y., Yang, N., Chen, X., Wen, L., Wang, X., Zhang, L., Ding, Y."Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments". International Journal of Molecular Medicine 31, no. 5 (2013): 1195-1201. https://doi.org/10.3892/ijmm.2013.1305
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